来自白腐真菌云芝的木质素过氧化物酶基因的克隆与特性分析
Cloning and characterization of a lignin peroxidase gene from the white-rot fungus Trametes versicolor.
作者信息
Black A K, Reddy C A
机构信息
Department of Microbiology and Public Health, Michigan State University, East Lansing 48824-1101.
出版信息
Biochem Biophys Res Commun. 1991 Aug 30;179(1):428-35. doi: 10.1016/0006-291x(91)91388-s.
Six putative lignin peroxidase (LIP) genes were isolated from a lambda EMBL3 phage library of the white-rot fungus, Trametes versicolor, using the Phanerochaete chrysosporium LIP cDNA CLG5 as the probe. Sequence analysis of one of the genes, VLG1, showed that its coding region is interrupted by six small introns (49-64 bp) and that it encodes a mature LIP protein (341 aa; Mr: 36,714) that is preceded by a 25 aa signal sequence. This protein has a relatively high degree of aa homology to the N-termini of the LIP proteins purified from T. versicolor and has an aa homology of 55-60% to the LIP proteins of P. chrysosporium, which is comparable to that found between P. chrysosporium and Phlebia radiata LIP proteins.
以黄孢原毛平革菌木质素过氧化物酶cDNA CLG5为探针，从白腐真菌云芝的λEMBL3噬菌体文库中分离出6个假定的木质素过氧化物酶（LIP）基因。对其中一个基因VLG1的序列分析表明，其编码区被6个小内含子（49 - 64 bp）中断，并且它编码一个成熟的LIP蛋白（341个氨基酸；分子量：36,714），该蛋白之前有一个25个氨基酸的信号序列。该蛋白与从云芝中纯化的LIP蛋白的N端具有较高的氨基酸同源性，与黄孢原毛平革菌的LIP蛋白具有55 - 60%的氨基酸同源性，这与黄孢原毛平革菌和辐射状韧革菌LIP蛋白之间的同源性相当。
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