糙皮侧耳利用玉米浆作为氮源和诱导物提高漆酶产量。
Enhanced laccase production by Trametes versicolor using corn steep liquor as both nitrogen source and inducer.
机构信息
National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, PR China.
National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, PR China.
出版信息
Bioresour Technol. 2014 Aug;166:602-5. doi: 10.1016/j.biortech.2014.05.068. Epub 2014 May 27.
A highly efficient strategy for laccase production by Trametes versicolor was developed using corn steep liquor (CSL) as both a nitrogen source and a laccase inducer. At the optimal CSL concentration of 20 gL(-1), an extracellular laccase activity of 633.3 UL(-1) was produced after a culture period of only 5 days. This represented a 1.96-fold increase relative to control medium lacking CSL. The addition of crude phenolic extracts from CSL improved laccase production to 91.8% greater than the control. Sinapinic acid, present in CSL, caused a reduction in laccase production, vanillic acid and ferulic acid (also present in CSL) synergistically induced laccase production by more than 100% greater than the control medium. Vanillic acid and ferulic acid provided the main contribution to the enhancement of laccase production. This study provides a basis for understanding the induction mechanism of CSL for laccase production.
采用玉米浆（CSL）作为氮源和漆酶诱导剂，开发了一种高效生产木聚糖酶的策略。在最佳的 CSL 浓度 20 gL(-1)下，培养 5 天后，可产生 633.3 UL(-1)的细胞外漆酶活性。与不含 CSL 的对照培养基相比，这一数值增加了 1.96 倍。CSL 中粗酚提取物的添加可将漆酶产量提高到比对照高 91.8%。CSL 中存在的咖啡酸会导致漆酶产量降低，香草酸和阿魏酸（也存在于 CSL 中）协同作用，使漆酶产量比对照培养基增加 100%以上。香草酸和阿魏酸对漆酶产量的提高起主要作用。本研究为理解 CSL 诱导漆酶产生的机制提供了依据。
Zotero 插件