The Institutes for Applied Research, Ben-Gurion University of the Negev, P.O.B. 1025, Beer-Sheva 84110, Israel.
Appl Environ Microbiol. 1988 Oct;54(10):2411-4. doi: 10.1128/aem.54.10.2411-2414.1988.
Porphyridium cultures grown on either nitrate or ammonium as the nitrogen source showed similar patterns of growth and cell wall polysaccharide production. The effect of nitrogen on growth and cell wall polysaccharide production was studied by applying three regimens of supply: batch mode, in which nitrate was supplied at the beginning of the experiment and became depleted at day 6; continual mode, in which nitrate was added daily; and deficient mode, in which the cells were cultured in a nitrate-free medium. Growth was similar in the batch- and continual-mode cultures, whereas it was totally inhibited in the deficient-mode culture. Polysaccharide content (per volume) was highest in the batch-mode culture and lowest in the deficient-mode culture. However, polysaccharide production per cell was similar in the continual- and deficient-mode cultures, the highest value being found in the batch-mode culture. In addition to its effect on polysaccharide content, nitrogen affected the polysaccharide distribution between soluble and bound polysaccharides. In the deficientmode culture, most of the cell wall polysaccharide was dissolved in the medium.
在硝酸盐或铵盐作为氮源的条件下培养的紫球藻表现出相似的生长和细胞壁多糖产生模式。通过应用三种供应方案研究了氮对生长和细胞壁多糖产生的影响：分批模式，其中在实验开始时添加硝酸盐，然后在第 6 天耗尽；连续模式，其中每天添加硝酸盐；以及缺乏模式，其中在无硝酸盐的培养基中培养细胞。在分批和连续模式培养中，生长相似，而在缺乏模式培养中，生长完全受到抑制。多糖含量（每体积）在分批模式培养中最高，在缺乏模式培养中最低。然而，每细胞的多糖产生在连续和缺乏模式培养中相似，最高值出现在分批模式培养中。除了对多糖含量的影响外，氮还影响多糖在可溶性和结合性多糖之间的分布。在缺乏模式培养中，大部分细胞壁多糖溶解在培养基中。
