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鸢尾黄素前体 D-异佛尔酮苷对 HepG2 细胞酒精性肝损伤的保护作用。

The Protective Effect of the Polysaccharide Precursor, D-Isofloridoside, from on Alcohol-Induced Hepatotoxicity in HepG2 Cells.

机构信息

College of Food Science and Technology, School of Chemistry and Environment, Guangdong Ocean University, Zhanjiang 524088, China.

Southern Marine Science and Engineering Guangdong Laboratory, Zhanjiang, 524025, China.

出版信息

Molecules. 2020 Feb 25;25(5):1024. doi: 10.3390/molecules25051024.

DOI:10.3390/molecules25051024
PMID:32106572
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7179215/
Abstract

Alcoholic liver disease (ALD) threatens human health, so it is imperative that we find ways to prevent or treat it. In recent years, the study of polysaccharides has shown that they have different kinds of bioactivities. Among them are many biological effects that have been attributed to polysaccharide precursors. D-Isofloridoside (DIF) is one of the polysaccharide precursors from the marine red alga . This study evaluated the effect of DIF on alcohol-induced oxidative stress in human hepatoma cells (HepG2). As a result, DIF attenuated alcohol-induced cytotoxicity, reduced the amount of intracellular reactive oxygen species (ROS), and effectively reduced alcohol-induced DNA damage in HepG2 cells. In addition, a western blot showed that, after DIF treatment, the expression levels of glutathione (GSH), superoxide dismutase (SOD), and B-cell lymphoma-2 (bcl-2) increased, while the expression levels of γ-glutamyl transferase (GGT), BCL2-associated X (bax), cleaved caspase-3, and mitogen-activated protein kinase (p38 and c-Jun N-terminal kinase ) signal transduction proteins reduced. This showed that DIF may protect cells by reducing the amount of intracellular ROS and inhibiting intracellular oxidative stress and apoptotic processes. Finally, molecular docking demonstrated that DIF can bind to SOD, GGT, B-cell lymphoma-, and bax proteins. These results indicated that DIF can protect HepG2 cells from alcohol-induced oxidative stress damage, making it an effective potential ingredient in functional foods.

摘要

酒精性肝病（ALD）威胁着人类健康，因此我们必须寻找预防或治疗它的方法。近年来，多糖的研究表明，它们具有不同种类的生物活性。其中许多生物效应归因于多糖前体。D-异佛尔酮糖苷（DIF）是海洋红藻多糖前体之一。本研究评估了 DIF 对人肝癌细胞（HepG2）中酒精诱导的氧化应激的影响。结果表明，DIF 减轻了酒精诱导的细胞毒性，减少了细胞内活性氧（ROS）的含量，并有效减少了 HepG2 细胞中酒精诱导的 DNA 损伤。此外，Western blot 显示，DIF 处理后，谷胱甘肽（GSH）、超氧化物歧化酶（SOD）和 B 细胞淋巴瘤-2（bcl-2）的表达水平增加，而 γ-谷氨酰转移酶（GGT）、B 细胞淋巴瘤-2 相关 X（bax）、裂解半胱天冬酶-3 和丝裂原活化蛋白激酶（p38 和 c-Jun N-末端激酶）信号转导蛋白的表达水平降低。这表明 DIF 可能通过减少细胞内 ROS 的含量并抑制细胞内氧化应激和凋亡过程来保护细胞。最后，分子对接表明 DIF 可以与 SOD、GGT、B 细胞淋巴瘤和 bax 蛋白结合。这些结果表明，DIF 可以保护 HepG2 细胞免受酒精诱导的氧化应激损伤，使其成为功能性食品中一种有效的潜在成分。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91bc/7179215/091616bd9eab/molecules-25-01024-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91bc/7179215/c0cfd07f6969/molecules-25-01024-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91bc/7179215/2f1a3abf2c55/molecules-25-01024-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91bc/7179215/f03806e78282/molecules-25-01024-g003a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91bc/7179215/572181cf12bf/molecules-25-01024-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91bc/7179215/fb28ee30a4de/molecules-25-01024-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91bc/7179215/1caa9995d2b2/molecules-25-01024-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91bc/7179215/091616bd9eab/molecules-25-01024-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91bc/7179215/c0cfd07f6969/molecules-25-01024-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91bc/7179215/2f1a3abf2c55/molecules-25-01024-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91bc/7179215/f03806e78282/molecules-25-01024-g003a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91bc/7179215/572181cf12bf/molecules-25-01024-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91bc/7179215/fb28ee30a4de/molecules-25-01024-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91bc/7179215/1caa9995d2b2/molecules-25-01024-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91bc/7179215/091616bd9eab/molecules-25-01024-g007.jpg

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