Department of Crop and Soil Sciences, Michigan State University, East Lansing, Michigan 48824-1325.
Appl Environ Microbiol. 1990 Apr;56(4):1198-201. doi: 10.1128/aem.56.4.1198-1201.1990.
Dehalogenation of carbon tetrachloride, chloroform, and bromoform in pure cultures of Methanosarcina sp. strain DCM and Methanosarcina mazei S6 was demonstrated. The initial dechlorination product of chloroform was methylene chloride (dichloromethane), which accumulated transiently to about 70% of the added chloroform; trace amounts of chloromethane were also detected. The amount of chloroform dechlorinated per mole of methane produced was approximately 10 times greater than the ratio observed previously for tetrachloroethene dechlorination by these strains. The production of CO(2) from [C]chloroform and the absence of CH(4) imply that processes in addition to reductive dechlorination operate.
产甲烷八叠球菌 DCM 株和产甲烷蘑菇 S6 纯培养物中可实现四氯化碳、氯仿和溴仿的脱卤反应。氯仿的初始脱卤产物是二氯甲烷（氯仿），其在添加的氯仿中短暂积累至约 70%；还检测到痕量的氯甲烷。每摩尔甲烷产生的氯仿脱卤量约为先前观察到的这些菌株对四氯乙烯脱卤反应的 10 倍。[C]氯仿生成 CO(2)和没有 CH(4)表明除了还原脱卤反应之外还有其他过程在起作用。
