用于检测聚丙烯酰胺凝胶中木聚糖酶活性的简单、灵敏的同工酶谱技术。
Simple, sensitive zymogram technique for detection of xylanase activity in polyacrylamide gels.
机构信息
Department of Environmental and Forest Biology, College of Environmental Science and Forestry, State University of New York, Syracuse, New York 13210.
出版信息
Appl Environ Microbiol. 1990 Jun;56(6):1516-7. doi: 10.1128/aem.56.6.1516-1517.1990.
Abstract
A method capable of detecting as little as 0.11 U of xylanase activity in polyacrylamide gels was developed. The method entails incubation of protein gels in contact with substrate gels containing unmodified xylan, followed by immersion of substrate gels in 95% ethanol. Resulting zymograms contain transparent bands corresponding to enzymatic activity against an opaque background.
摘要
开发了一种能够在聚丙烯酰胺凝胶中检测低至 0.11 U 的木聚糖酶活性的方法。该方法包括将蛋白质凝胶与含有未修饰木聚糖的底物凝胶接触孵育，然后将底物凝胶浸入 95%乙醇中。得到的酶谱含有对应于对不透明背景的酶活性的透明带。
Zotero 插件