Departments of Applied Biology and Chemistry, University of Hull, Hull HU6 7RX, United Kingdom.
Appl Environ Microbiol. 1990 Nov;56(11):3354-9. doi: 10.1128/aem.56.11.3354-3359.1990.
A number of Pseudomonas species have been identified which accumulate a polyhydroxyalkanoate containing mainly 3-hydroxydecanoate monomers from sodium gluconate as the sole carbon source. One of these, Pseudomonas sp. strain NCIMB 40135, was further investigated and shown to accumulate such a polyhydroxyalkanoate from a wide range of carbon sources (C(2) to C(6)); however, when supplied with octanoic acid it produced a polyhydroxyalkanoate containing mainly 3-hydroxyoctanoate monomers. Polymer synthesis occurred in batch culture after cessation of growth due to exhaustion of nitrogen. In continuous culture under nitrogen limitation up to 16.9% (wt/wt) polyhydroxyalkanoate was synthesized from glucose as the carbon source. The monomer units are mainly of the R-(-) configuration. Nuclear magnetic resonance studies confirmed the composition of the polymer. Differential scanning calorimetry suggested that the solvent-extracted polymer contained a significant proportion of crystalline material. The weight-average molecular weight of the polymer from glucose-grown cells was 143,000.
已鉴定出多种假单胞菌属物种，它们能够从葡萄糖酸钠作为唯一碳源积累主要含有 3-羟基癸酸单体的聚羟基烷酸酯。其中一种，假单胞菌属菌株 NCIMB 40135 进一步被研究，表明其可以从广泛的碳源（C2 到 C6）中积累这种聚羟基烷酸酯；然而，当供应辛酸时，它会产生主要含有 3-羟基辛酸单体的聚羟基烷酸酯。由于氮的耗尽，在停止生长后的间歇培养中发生聚合物合成。在氮限制的连续培养中，从葡萄糖作为碳源合成了高达 16.9%（wt/wt）的聚羟基烷酸酯。单体单元主要为 R-(-)构型。核磁共振研究证实了聚合物的组成。差示扫描量热法表明，溶剂提取的聚合物含有相当比例的结晶材料。来自葡萄糖生长细胞的聚合物的重均分子量为 143,000。
