Lehrstuhl für Mikrobiologie, Universität Bayreuth, D-8580 Bayreuth, and Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, D-3300 Braunschweig 2, Federal Republic of Germany.
Appl Environ Microbiol. 1990 Dec;56(12):3727-34. doi: 10.1128/aem.56.12.3727-3734.1990.
The novel thermophilic CO- and H(2)-oxidizing bacterium UBT1 has been isolated from the covering soil of a burning charcoal pile. The isolate is gram positive and obligately chemolithoautotrophic and has been named Streptomyces thermoautotrophicus on the basis of G+C content (70.6 +/- 0.19 mol%), a phospholipid pattern of type II, MK-9(H(4)) as the major quinone, and other chemotaxonomic and morphological properties. S. thermoautotrophicus could grow with CO (t(d) = 8 h), H(2) plus CO(2) (t(d) = 6 h), car exhaust, or gas produced by the incomplete combustion of wood. Complex media or heterotrophic substrates such as sugars, organic acids, amino acids, and alcohols did not support growth. Molybdenum was required for CO-autotrophic growth. For growth with H(2), nickel was not necessary. The optimum growth temperature was 65 degrees C; no growth was observed below 40 degrees C. However, CO-grown cells were able to oxidize CO at temperatures of 10 to 70 degrees C. Temperature profiles of burning charcoal piles revealed that, up to a depth of about 10 to 25 cm, the entire covering soil provides a suitable habitat for S. thermoautotrophicus. The K(m) was 88 mul of CO liter and V(max) was 20.2 mul of CO h mg of protein. The threshold value of S. thermoautotrophicus of 0.2 mul of CO liter was similar to those of various soils. The specific CO-oxidizing activity in extracts with phenazinemethosulfate plus 2,6-dichlorophenolindophenol as electron acceptors was 246 mumol min mg of protein. In exception to other carboxydotrophic bacteria, S. thermoautotrophicus CO dehydrogenase was able to reduce low potential electron acceptors such as methyl and benzyl viologens.
新型嗜热 CO 和 H(2)氧化细菌 UBT1 是从燃烧木炭堆的覆盖土壤中分离出来的。该分离株为革兰氏阳性，严格的化能自养型，根据 G+C 含量（70.6 +/- 0.19 mol%）、II 型磷脂模式、MK-9(H(4))作为主要醌和其他化学分类学和形态学特性，被命名为嗜热链霉菌。S. thermoautotrophicus 可以用 CO（t(d) = 8 h）、H(2)加 CO(2)（t(d) = 6 h）、汽车尾气或不完全燃烧木材产生的气体生长。复杂的培养基或异养底物，如糖、有机酸、氨基酸和醇，不能支持生长。钼是 CO 自养生长所必需的。对于 H(2)的生长，镍不是必需的。最适生长温度为 65 摄氏度；在 40 摄氏度以下没有观察到生长。然而，CO 培养的细胞能够在 10 到 70 摄氏度的温度下氧化 CO。燃烧木炭堆的温度曲线表明，在深度约 10 到 25 厘米范围内，整个覆盖土壤为 S. thermoautotrophicus 提供了一个适宜的栖息地。K(m)为 88 mul 的 CO liter，V(max)为 20.2 mul 的 CO h mg 的蛋白质。S. thermoautotrophicus 的阈值为 0.2 mul 的 CO liter，与各种土壤的阈值相似。用 phenazinemethosulfate 和 2,6-dichlorophenolindophenol 作为电子受体的提取物中的特定 CO 氧化活性为 246 mumol min mg 的蛋白质。除了其他羧化细菌外，S. thermoautotrophicus CO 脱氢酶还能够还原低电位电子受体，如甲基和苄基紫精。
