用于细菌全细胞脂肪酸甲酯（包括羟基酸）常规分析的单一衍生化方法。
Single derivatization method for routine analysis of bacterial whole-cell fatty acid methyl esters, including hydroxy acids.
作者信息
Miller L T
出版信息
J Clin Microbiol. 1982 Sep;16(3):584-6. doi: 10.1128/jcm.16.3.584-586.1982.
Abstract
Analysis of fatty acid methyl esters prepared from whole-cell bacteria by sodium hydroxide hydrolysis and boron trichloride-catalyzed methylation showed degradation of hydroxy acid peaks after several injections on a fused silica capillary column. A simple base wash of the extracts before injection prevented the tailing of the hydroxy acid peaks even after extended use. This eliminates the need to form trifluoroacetic anhydride derivatives of the hydroxy acids.
摘要
通过氢氧化钠水解和三氯化硼催化甲基化从全细胞细菌制备的脂肪酸甲酯分析表明，在熔融石英毛细管柱上多次进样后，羟基酸峰出现降解。进样前对提取物进行简单的碱洗，即使在长时间使用后也能防止羟基酸峰拖尾。这消除了形成羟基酸的三氟乙酸酐衍生物的必要性。
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