蓝藻的分离和纯化方法。
Method for isolation and purification of cyanobacteria.
机构信息
Department of Fermentation Microbiology, Merck Sharp & Dohme Research Laboratories, Rahway, New Jersey 07065.
出版信息
Appl Environ Microbiol. 1991 May;57(5):1448-52. doi: 10.1128/aem.57.5.1448-1452.1991.
Abstract
A method employing nutrient saturated glass fiber filters allowed the isolation of the same numbers of cyanobacteria from freshwater as were obtained with medium solidified with agar, while providing a 2- to 15-fold reduction in the number of accompanying heterotrophic bacteria. Imipenem, a broad-spectrum beta-lactam antibiotic which inhibits peptidoglycan biosynthesis, was superior to some other beta-lactam antibiotics for reducing the numbers of heterotrophic bacterial contaminants associated with freshly isolated cyanobacteria to a level which facilitated the production of axenic cyanobacterial cultures.
摘要
一种采用营养饱和玻璃纤维滤器的方法可从淡水中分离出与用琼脂固化的培养基相同数量的蓝藻，同时将伴随的异养细菌数量减少 2 至 15 倍。亚胺培南是一种广谱β-内酰胺抗生素，可抑制肽聚糖的生物合成，优于其他一些β-内酰胺抗生素，可将与新分离的蓝藻相关的异养细菌污染物的数量减少到便于生产无菌蓝藻培养物的水平。
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