Guevara Govinda, Espinoza Solorzano Jamileth Stefania, Vargas Ramírez Marta, Rusu Andrada, Navarro Llorens Juana María
Department of Biochemistry and Molecular Biology, Universidad Complutense de Madrid, c/Jose Antonio Novais 12, 28040 Madrid, Spain.
Department of Genetics, Physiology and Microbiology, Universidad Complutense de Madrid, c/Jose Antonio Novais 12, 28040 Madrid, Spain.
Int J Mol Sci. 2024 Dec 4;25(23):13018. doi: 10.3390/ijms252313018.
Microalga-bacteria consortia are increasingly recognized for their effectiveness in wastewater treatment, leveraging the metabolic synergy between microalgae and bacteria to enhance nutrient removal and overall treatment efficiency. These systems offer a sustainable approach to addressing pollutants such as nitrogen and phosphorus. However, their potential in removing specific contaminants like steroid hormones is less explored. In this study, a natural microbial consortium, A21, has been characterized and isolated from primary sewage treatment in Madrid and its potential for bioremediation of steroid hormone effluents has been evaluated. The A21 consortium includes Alphaproteobacteria genera and and the Cyanobacterium . (31.78%) is known for biodegradation, while (15.68%) exhibits detoxification abilities. (14.2%) may contribute to nutrient uptake and oxygen production. The effects of pH, nitrogen sources, and Sodium chloride concentrations on growth were evaluated. The optimal growth conditions were determined to be a pH range of 7 to 9, a salt concentration below 0.1 M, and the presence of a nitrogen source. The consortium also demonstrated effective growth across various types of wastewaters (primary, secondary, and tertiary treatment effluents). Additionally, A21 exhibited the ability to grow in the presence of steroids and transform them into other compounds, such as converting androstenedione (AD) into androsta-1,4-diene-3,17-dione (ADD) and β-estradiol into estrone.
微藻-细菌共生体因其在废水处理中的有效性而越来越受到认可，它利用微藻和细菌之间的代谢协同作用来提高养分去除率和整体处理效率。这些系统为解决氮和磷等污染物提供了一种可持续的方法。然而，它们在去除类固醇激素等特定污染物方面的潜力尚未得到充分探索。在本研究中，从马德里的一级污水处理中鉴定并分离出一种天然微生物共生体A21，并评估了其对类固醇激素废水的生物修复潜力。A21共生体包括α-变形杆菌属和蓝藻。（31.78%）以生物降解而闻名，而（15.68%）具有解毒能力。（14.2%）可能有助于养分吸收和氧气产生。评估了pH值、氮源和氯化钠浓度对生长的影响。确定最佳生长条件为pH值范围为7至9、盐浓度低于0.1 M以及存在氮源。该共生体在各种类型的废水（一级、二级和三级处理出水）中也表现出有效的生长。此外，A21在类固醇存在的情况下具有生长能力，并能将它们转化为其他化合物，例如将雄烯二酮（AD）转化为雄甾-1,4-二烯-3,17-二酮（ADD）以及将β-雌二醇转化为雌酮。
