含有与土壤溶液中营养离子浓度相似的新型培养基。
New culture medium containing ionic concentrations of nutrients similar to concentrations found in the soil solution.
机构信息
Department of Agronomy, University of Maryland, College Park, Maryland 20742; Soils and Agronomy Department, AFRC Institute of Arable, Crops Research, Rothamsted Experimental Station, Harpenden, Hertfordshire AL5 2JQ, United Kingdom ; and Environmental Chemistry Laboratory, Agricultural Research Service, U.S. Department of Agriculture, Beltsville, Maryland 20705.
出版信息
Appl Environ Microbiol. 1991 Dec;57(12):3674-6. doi: 10.1128/aem.57.12.3674-3676.1991.
Abstract
A new growth medium which closely approximates the composition of the soil solution is presented. This soil solution equivalent (SSE) medium contains the following components (millimolar): NO(3), 2.5; NH(4), 2.5; HPO(4), 0.005; Na, 2.5; Ca, 4.0; Mg, 2.0; K, 0.503; Cl, 4.0; SO(4), 5.0; ethylenediamine-di(o-hydroxyphenylacetic acid), 0.02; and MES [2-(N-morpholino)ethanesulfonic acid] (to maintain the pH at 6.0), 10, plus 0.1% arabinose. The advantages of the SSE medium are discussed.
摘要
现提出一种新的生长培养基，其成分非常接近土壤溶液的组成。这种土壤溶液当量（SSE）培养基含有以下成分（毫摩尔）：NO3，2.5；NH4，2.5；HPO4，0.005；Na，2.5；Ca，4.0；Mg，2.0；K，0.503；Cl，4.0；SO4，5.0；乙二胺二（邻羟苯基乙酸），0.02；MES[2-（N-吗啉基）乙磺酸]（维持 pH 值在 6.0），10，外加 0.1%阿拉伯糖。讨论了 SSE 培养基的优点。
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