sp. nov., a new acidophilic representative of the isolated from fen soils.

Two new strains JP48 and JP55 affiliated with the acidobacterial class have been isolated from fen soil sampled in the Fichtelgebirge Mountains near Bayreuth, Germany. Both strains were Gram-stain-negative, non-motile, non-spore-forming rods that divide by binary fission, segregate exopolysaccharide-like material and form capsules. Strains JP48 and JP55 grew at 4-36 °C (optimum at 27 °C), pH 3.6-7.3 (optimum at pH 4.6-5.5) and with NaCl concentrations of 0-3% (optimum at 1.0%; w/v). Strains JP48 and JP55 grew aerobically on a wide range of organic substrates including mono- and oligosaccharides, amino acids and short-chained fatty acids. MK-8 was identified as the major respiratory quinone. The major fatty acids for strains JP48 and JP55 were -C, C ω7c, C and -diabolic acid. Phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol, lysophophatidylethanolamine, phosphatidylcholine, unidentified glyco- and glycophospholipids, and unidentified high mass lipid species were the major polar membrane lipids. The G+C content of strains JP48 and JP55 was 57.4 and 57.2 mol%, respectively. The genomes of strains JP48 and JP55 contained nine potential secondary metabolite regions encoding for the compound classes NRPS(-like), T3PKS, terpene, or lanthipeptide class IV. Phylogenetic reconstruction and 16S rRNA gene sequence similarities of 98.3 and 96.9% identified DHF9 and DSM 104462 as the most closely related type strains to strains JP48 and JP55. Based on their phenotype, phylogeny and chemotaxonomy, we propose the novel species sp. nov. (type strain JP48=DSM 109919=CECT 30269; additional strain JP55=DSM 109920=CECT 30268) within the class of the phylum .