纳他霉素作为琼脂培养基中的一种杀菌剂。
Natamycin as a fungicide in agar media.
机构信息
Department of Marine Ecology and Microbiology, National Environmental Research Institute, Frederiksborgvej 399, P.O. Box 358, DK-4000 Roskilde, Denmark.
出版信息
Appl Environ Microbiol. 1992 Mar;58(3):1064-6. doi: 10.1128/aem.58.3.1064-1066.1992.
Abstract
Fungal inhibition in four commonly used agar media was improved by substituting natamycin (pimaricin) for cycloheximide. The recovery of bacteria was not affected by natamycin, whereas fungal contamination from a variety of samples was significantly suppressed. Furthermore, natamycin lacks the occupational health hazards of cycloheximide. Medium-dependent natamycin degradation occurred during the preparation and refrigerated storage of agar plates, but the addition of natamycin at 21.6 mug/ml resulted in effective residual activity.
摘要
在四种常用的琼脂培养基中，通过用纳他霉素（匹马霉素）替代环己酰亚胺来提高真菌抑制作用。纳他霉素对细菌的回收没有影响，而各种样品的真菌污染则得到了显著抑制。此外，纳他霉素没有环己酰亚胺的职业健康危害。在琼脂平板的制备和冷藏储存过程中，纳他霉素会发生介质依赖性降解，但添加 21.6 微克/毫升的纳他霉素可产生有效的残留活性。
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