纳他霉素作为军团菌属生长培养基中的一种选择性抗真菌剂
Natamycin as a selective antifungal agent in media for growth of Legionella spp.
作者信息
Edelstein P H, Edelstein M A
机构信息
Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia 19104-4283, USA. phe@leland. stanford.edu
出版信息
J Clin Microbiol. 1996 Jan;34(1):185-7. doi: 10.1128/jcm.34.1.185-187.1996.
The growth of 18 different Legionella sp. strains and 76 different yeast isolates was tested on buffered charcoal yeast extract medium supplemented with alpha-ketoglutarate (BCYE alpha medium) and with natamycin, an antifungal agent. Bacterial growth was no different on BCYE alpha medium made with or without natamycin, whereas complete inhibition of yeasts occurred in BCYE alpha medium containing 200 to 500 micrograms of natamycin per ml. Selective BCYE alpha media made with natamycin rather than anisomycin had no (formulation with vancomycin, polymyxin B, and agar) or little (formulation with cefamandole, polymyxin B, and agar) inhibitory effect on the growth of 14 different Legionella sp. bacteria. Natamycin is an inexpensive alternative to anisomycin in the formulation of selective BCYE alpha media.
在添加了α-酮戊二酸的缓冲活性炭酵母提取物培养基（BCYEα培养基）以及添加了抗真菌剂那他霉素的培养基上，测试了18种不同军团菌属菌株和76种不同酵母分离株的生长情况。在添加或不添加那他霉素的BCYEα培养基上，细菌生长没有差异，而在每毫升含有200至500微克那他霉素的BCYEα培养基中，酵母生长被完全抑制。用那他霉素而非茴香霉素制成的选择性BCYEα培养基对14种不同军团菌属细菌的生长没有（含万古霉素、多粘菌素B和琼脂的配方）或几乎没有（含头孢孟多、多粘菌素B和琼脂的配方）抑制作用。在选择性BCYEα培养基的配方中，那他霉素是茴香霉素的一种廉价替代品。
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