Matsunaga R, Sawamura K, De Kok M, Makino T, Miki K, Kojima M, Tsuchizaki T, Hibi T
Laboratory of Plant Pathology, Faculty of Agriculture, University of Tokyo, Japan.
J Gen Virol. 1992 Apr;73 ( Pt 4):763-6. doi: 10.1099/0022-1317-73-4-763.
Protoplasts isolated from tomato, wild tomato, barley and chrysanthemum were electrotransfected with tobacco mosaic virus (TMV) RNA under almost the same optimum electric conditions: five square DC pulses of 50 microseconds duration at 500 to 800 V/cm, with the protoplasts suspended at 2 x 10(5)/ml in 0.5 M-mannitol containing 100 microM-MgCl2 and 10 to 20 micrograms/ml TMV RNA. ELISAs of these transfected protoplasts showed that the yields and the growth curves of the virus were quite similar, indicating a lack of host specificity in the initially infected cells of these plants.
