Laboratorio de Bioquímica, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Casilla 114-D, Santiago, Chile, and Institute for Microbial and Biochemical Technology, Forest Products Laboratory, Forest Service, U.S. Department of Agriculture, Madison, Wisconsin 53705-2398.
Appl Environ Microbiol. 1993 Jun;59(6):1792-7. doi: 10.1128/aem.59.6.1792-1797.1993.
The ability of the white rot fungus Ceriporiopsis subvermispora to mineralize C-synthetic lignin was studied under different culture conditions, and the levels of two extracellular enzymes were monitored. The highest mineralization rates (28% after 28 days) were obtained in cultures containing a growth-limiting amount of nitrogen source (1.0 mM ammonium tartrate); under this condition, the levels of manganese peroxidase (MnP) and laccase present in the culture supernatant solutions were very low compared with cultures containing 10 mM of the nitrogen source. In contrast, cultures containing a limiting concentration of the carbon source (0.1% glucose) showed low levels of both enzymes and also very low mineralization rates compared with cultures containing 1% glucose. Cultures containing 11 ppm of Mn(II) showed a higher rate of mineralization than those containing 0.3 or 40 ppm of this cation. Levels of MnP and laccase were higher when 40 ppm of Mn(II) was used. Mineralization rates were slightly higher in cultures flushed daily with oxygen, whereas laccase levels were lower and MnP levels were approximately the same as in cultures maintained under an air atmosphere. The presence of 0.4 mM veratryl alcohol reduced both mineralization rates and MnP levels, without affecting laccase levels. Lignin peroxidase activity was not detected under any condition. Addition of purified lignin peroxidase to the cultures in the presence or absence of veratryl alcohol did not enhance mineralization rates significantly.
白腐菌 Ceriporiopsis subvermispora 对 C 型合成木质素的矿化能力在不同的培养条件下进行了研究，并监测了两种胞外酶的水平。在含有生长限制氮源（1.0mM 酒石酸铵）的培养物中获得了最高的矿化率（28%，28 天后）；在这种条件下，与含有 10mM 氮源的培养物相比，培养物上清液中锰过氧化物酶（MnP）和漆酶的水平非常低。相比之下，在含有限制浓度碳源（0.1%葡萄糖）的培养物中，两种酶的水平都很低，矿化率也很低，与含有 1%葡萄糖的培养物相比。与含有 0.3 或 40ppm 这种阳离子的培养物相比，含有 11ppmMn(II)的培养物显示出更高的矿化率。当使用 40ppmMn(II)时，MnP 和漆酶的水平更高。每天用氧气冲洗培养物时，矿化率略高，而漆酶水平较低，MnP 水平与在空气气氛下维持的培养物大致相同。存在 0.4mM 藜芦醇会降低矿化率和 MnP 水平，而不会影响漆酶水平。在任何条件下都未检测到木质素过氧化物酶活性。在有或没有藜芦醇醇的情况下向培养物中添加纯化的木质素过氧化物酶并没有显著提高矿化率。
