Afrida Sitompul, Tamai Yutaka, Watanabe Toshihiro, Osaki Mitsuru
Laboratory of Plant Nutrition, Graduate School of Agriculture, Hokkaido University, N9W9, Kita-ku, Sapporo, 060-8589, Japan.
World J Microbiol Biotechnol. 2014 Aug;30(8):2263-71. doi: 10.1007/s11274-014-1647-7. Epub 2014 Apr 4.
The white-rot fungi Irpex lacteus KB-1.1 and Lentinus tigrinus LP-7 have been shown in previous studies to have high biobleaching activity in vivo. The aim of this study was to investigate the activities and stabilities of extracellular enzymes, prepared from I. lacteus and L. tigrinus culture grown in three types of economical media of agricultural and forestry wastes, for biobleaching of Acacia oxygen-delignified kraft pulp using kappa number reduction as an indicator of delignification. After 3 days of incubation, the extracellular enzymes preparations from I. lacteus and L. tigrinus cultures in media of Acacia mangium wood powder supplemented with rice bran and addition 1 % glucose (WRBG), resulted in significant decrease of 4.4 and 6.7 %, respectively. A slightly higher kappa number reduction (7.4 %) was achieved with the combine extracellular enzymes from I. lacteus and L. tigrinus. One of the strategies for reducing the cost of enzyme production for treatment processes in the pulp and paper industry is the utilization of agricultural and forestry waste. Thus, WRBG has potential as a culture medium for producing stable lignolytic enzymes simply and economically.
先前的研究表明，白腐真菌乳白耙齿菌KB-1.1和虎皮香菇LP-7在体内具有较高的生物漂白活性。本研究的目的是研究从在三种农林废弃物经济培养基中培养的乳白耙齿菌和虎皮香菇制备的胞外酶对相思木氧脱木素硫酸盐浆进行生物漂白的活性和稳定性，以卡伯值降低作为脱木素的指标。培养3天后，在添加米糠和1%葡萄糖的马占相思木粉培养基（WRBG）中培养的乳白耙齿菌和虎皮香菇的胞外酶制剂分别使卡伯值显著降低了4.4%和6.7%。乳白耙齿菌和虎皮香菇的复合胞外酶使卡伯值降低得稍高一些（7.4%）。降低制浆造纸工业处理过程中酶生产成本的策略之一是利用农林废弃物。因此，WRBG有潜力作为一种简单且经济地生产稳定木质素分解酶的培养基。
