pH 值和温度对中温和碱性冰岛温泉中纤维素和半纤维素降解嗜热厌氧菌计数的影响。
Influence of pH and Temperature on Enumeration of Cellulose- and Hemicellulose-Degrading Thermophilic Anaerobes in Neutral and Alkaline Icelandic Hot Springs.
机构信息
Department of Biotechnology, Building 223, Technical University of Denmark, DK-2800 Lyngby, Denmark, and Biotechnology Department, Technological Institute of Iceland, Keldnaholt, IS-112 Reykjavik, Iceland.
出版信息
Appl Environ Microbiol. 1993 Jun;59(6):1963-5. doi: 10.1128/aem.59.6.1963-1965.1993.
Cellulose- and hemicellulose-degrading thermophilic anaerobes were enumerated in biomat samples of various temperatures from two different hot springs in the Hverageroi area of Iceland: one spring had a pH near 7, the second had a pH near 9. The most-probable-number technique was used for enumeration of bacteria in the samples, with media at many different temperatures (37 to 90 degrees C) and two pH values (7 and 9). There were generally more xylan-degrading then cellulose-utilizing organisms in both environments. There was no growth at 80 degrees C in the neutral spring or at 37 degrees C in the alkaline spring. However, there were large numbers of both types of organisms in the alkaline spring at 80 degrees C and in the neutral spring at 37 degrees C. No cultures grew from the most-probable-number tubes inoculated with the Hverageroi samples and incubated at 90 degrees C or with media at pH 9. However, xylan-degrading cultures at 70 degrees C were enriched at pH 9 with samples from some other Icelandic hot springs.
在冰岛赫瓦雷焦伊地区的两个不同温泉的生物基质样本中，对纤维素和半纤维素降解的嗜热厌氧菌进行了计数：一个温泉的 pH 值接近 7，另一个温泉的 pH 值接近 9。使用最可能数技术对样品中的细菌进行了计数，使用了许多不同温度（37 至 90°C）和两种 pH 值（7 和 9）的培养基。在这两种环境中，通常有更多的木聚糖降解菌而不是纤维素利用菌。中性温泉中 80°C 时没有生长，碱性温泉中 37°C 时也没有生长。然而，碱性温泉中 80°C 和中性温泉中 37°C 时，这两种类型的生物数量都很多。从赫瓦雷焦伊样品接种的最可能数管中没有培养物在 90°C 或 pH 值为 9 的培养基中生长。然而，用来自其他一些冰岛温泉的样品在 pH 值为 9 时在 70°C 下富集了木聚糖降解培养物。
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