Bredholt S, Mathrani I M, Ahring B K
Institute of Environmental Science and Engineering, Technical University of Denmark, Lyngby.
Antonie Van Leeuwenhoek. 1995 Nov;68(4):263-71. doi: 10.1007/BF00874135.
Anaerobic enrichment cultures with Avicel as substrate and inoculated with biomat samples from Icelandic hot springs were cultured at 70 degrees C or 78 degrees C and examined for the presence of microorganisms that produce extracellular cellulolytic and xylanolytic enzymes. From four enrichments grown at 78 degrees C eighteen strains were isolated. Five of the strains were screened for their substrate utilization, and on the basis of differences in morphology and substrates used, the two most unique strains were selected for further characterization. All cellulolytic cultures were rod-shaped and non-sporeforming. Motility was not observed. Cells stained gram-negative at various stages of the growth phase. During growth on Avicel, most cultures produced acetate as the major fermentation product, with smaller amounts of lactic acid and ethanol. Carbon dioxide and hydrogen were also produced. The phenotypic characteristics of the enrichment cultures and of isolates are described and assessed in relation to temperature and pH in the hot spring environment. A comparison is made between Icelandic strains isolated in our laboratory and strains isolated from hot springs from other parts of the world. The biotechnological potential of this group of bacteria is briefly discussed.
以微晶纤维素为底物，接种来自冰岛温泉的生物膜样品，进行厌氧富集培养，在70摄氏度或78摄氏度下培养，并检测产生细胞外纤维素酶和木聚糖酶的微生物的存在情况。从在78摄氏度下生长的四个富集培养物中分离出18株菌株。对其中5株菌株的底物利用情况进行了筛选，并根据形态和所用底物的差异，选择了两个最独特的菌株进行进一步鉴定。所有纤维素分解培养物均为杆状且不形成芽孢。未观察到运动性。细胞在生长阶段的不同时期革兰氏染色均为阴性。在微晶纤维素上生长期间，大多数培养物产生乙酸作为主要发酵产物，同时产生少量乳酸和乙醇。还产生二氧化碳和氢气。描述并评估了富集培养物和分离株的表型特征与温泉环境中的温度和pH的关系。对我们实验室分离的冰岛菌株与世界其他地区温泉分离的菌株进行了比较。简要讨论了这组细菌的生物技术潜力。
