Schraa G, Boone M L, Jetten M S, van Neerven A R, Colberg P J, Zehnder A J
Appl Environ Microbiol. 1986 Dec;52(6):1374-81. doi: 10.1128/aem.52.6.1374-1381.1986.
An organism, identified as an Alcaligenes sp., was isolated from an enrichment culture in which 1,4-dichlorobenzene served as the sole carbon and energy source. During growth with 1,4-dichlorobenzene in pure culture, stoichiometric amounts of chloride were released. Growth experiments and oxygen uptake rates with other chlorinated aromatic compounds revealed a high degree of specificity of the initial dioxygenase. cis-1,2-Dihydroxycyclohexa-3,5-diene oxidoreductase and 1,2-pyrocatechase, but not 2,3-pyrocatechase, were found in cell extracts, while 3,6-dichlorocatechol and (2,5-dichloro)muconic acid could be detected as intermediates during degradation of 1,4-dichlorobenzene. It is proposed that dioxygenases are involved in the initial steps of 1,4-dichlorobenzene degradation, while ring opening proceeds via ortho cleavage.
从一种富集培养物中分离出一种被鉴定为产碱菌属的微生物，在该培养物中1,4 - 二氯苯作为唯一的碳源和能源。在纯培养物中以1,4 - 二氯苯生长期间，化学计量的氯离子被释放。用其他氯代芳香化合物进行的生长实验和氧摄取率表明初始双加氧酶具有高度特异性。在细胞提取物中发现了顺式 - 1,2 - 二羟基环己 - 3,5 - 二烯氧化还原酶和1,2 - 邻苯二酚酶，但未发现2,3 - 邻苯二酚酶，而在1,4 - 二氯苯降解过程中可检测到3,6 - 二氯邻苯二酚和（2,5 - 二氯）粘康酸作为中间产物。有人提出双加氧酶参与1,4 - 二氯苯降解的初始步骤，而开环通过邻位裂解进行。
