Institute of Animal Physiology and Genetics, Czech Academy of Sciences, 104 00 Prague 10, Uhríneves, Czech Republic.
Appl Environ Microbiol. 1994 Feb;60(2):738-9. doi: 10.1128/aem.60.2.738-739.1994.
The dual-substrate utilization pattern in cultures of five ruminal strains of Butyrivibrio fibrisolvens growing on glucose and xylose was investigated. Strains ATCC 19171 and 86 utilized glucose and xylose simultaneously. Other strains exhibited diauxic growth. Strains X1 and CE 51 exhibited classical diauxic growth in which glucose was utilized during the first phase. Strain X2D62 displayed atypical diauxic growth in which slow utilization of xylose was followed by rapid utilization of glucose after the xylose depletion. The ATP-dependent phosphorylation of glucose was found in all strains tested. The phosphoenolpyruvate-dependent phosphorylation of glucose was detected only in B. fibrisolvens CE 51.
研究了五种纤维丁酸弧菌(Butyrivibrio fibrisolvens)瘤胃液菌株在葡萄糖和木糖上的双重底物利用模式。菌株 ATCC 19171 和 86 同时利用葡萄糖和木糖。其他菌株表现出双相生长。菌株 X1 和 CE51 表现出典型的双相生长,其中葡萄糖在第一阶段被利用。菌株 X2D62 表现出非典型的双相生长,木糖缓慢利用后,木糖耗尽后迅速利用葡萄糖。所有测试菌株都发现了依赖于 ATP 的葡萄糖磷酸化。只有在纤维丁酸弧菌 CE51 中检测到依赖磷酸烯醇丙酮酸的葡萄糖磷酸化。