Lehrstuhl für Okologische Mikrobiologie, BITOK, Universität Bayreuth, 95440 Bayreuth, Germany.
Appl Environ Microbiol. 1994 Apr;60(4):1370-3. doi: 10.1128/aem.60.4.1370-1373.1994.
Soil obtained from a beech forest formed significant amounts of acetate when incubated in a bicarbonate-buffered, mineral salt solution under anaerobic conditions at both 5 and 20 degrees C (21 and 38 g of acetate per kg [dry weight] of soil, respectively). At 20 degrees C, following an 18-day lag period, rates of 0.07 mmol of acetate synthesized per g (dry weight) of soil per day were observed. Acetate was not subject to immediate turnover; methane and hydrogen were not formed during the time intervals (5 degrees C, 335 days; 20 degrees C, 95 days) evaluated. The synthesis of acetate from endogenous materials was coincident with acetogenic potentials, i.e., the capacity to catalyze the H(2)-dependent synthesis of acetate. Hydrogen consumption was not directed towards the synthesis of methane. Collectively, these results suggest that acetogenesis may be an underlying microbial activity of this forest soil.
从山毛榉林中获取的土壤在 5℃和 20℃、无氧条件下、碳酸氢盐缓冲的矿盐溶液中培养时,生成了大量的乙酸盐(分别为每公斤(干重)土壤 21 和 38 克乙酸盐)。在 20℃下,经过 18 天的滞后期,观察到每天每克(干重)土壤合成 0.07 毫摩尔乙酸盐的速率。乙酸盐不会立即转化;在评估的时间间隔内(5℃,335 天;20℃,95 天)未形成甲烷和氢气。从内源性物质合成乙酸盐与产乙酸菌潜能一致,即催化 H2 依赖型乙酸盐合成的能力。氢气消耗未用于甲烷合成。总的来说,这些结果表明产乙酸作用可能是该森林土壤的一种潜在微生物活动。