Kellogg Biologícal Station, Michigan State University, Hickory Corners, Michigan 49060, and Department of Microbiology and Public Health, Michigan State University, East Lansing, Michigan 48824.
Appl Environ Microbiol. 1983 Apr;45(4):1310-5. doi: 10.1128/aem.45.4.1310-1315.1983.
C-tracer techniques were used to examine the metabolism of methanol and methylamines and acetogenesis from hydrogen and carbon dioxide in sediments from the profundal and littoral zones of eutrophic Wintergreen Lake, Michigan. Methanogens were primarily responsible for the metabolism of methanol, monomethylamine, and trimethylamine and maintained the pool size of these substrates below 10 muM in both sediment types. Methanol and methylamines were the precursors for less than 5 and 1%, respectively, of the total methane produced. Methanol and methylamines continued to be metabolized to methane when the sulfate concentration in the sediment was increased to 20 mM. Less than 2% of the total acetate production was derived from carbon dioxide reduction. Hydrogen consumption by hydrogen-oxidizing acetogens was 5% or less of the total hydrogen uptake by acetogens and methanogens. These results, in conjunction with previous studies, emphasize that acetate and hydrogen are the major methane precursors and that methanogens are the predominant hydrogen consumers in the sediments of this eutrophic lake.
C-示踪技术被用于研究密歇根州冬季绿湖深水区和浅水区沉积物中甲醇和甲基胺的代谢以及氢气和二氧化碳生成乙酸的过程。产甲烷菌主要负责甲醇、单甲基胺和三甲胺的代谢，并将这些基质的池大小维持在两种沉积物类型中均低于 10 μM。甲醇和甲基胺分别为产生的总甲烷的不到 5%和 1%的前体。当沉积物中的硫酸盐浓度增加到 20 mM 时，甲醇和甲基胺继续代谢为甲烷。总乙酸产量中不到 2%来自二氧化碳还原。氢氧化乙酸菌消耗的氢气占乙酸菌和产甲烷菌总氢气吸收量的 5%或更少。这些结果与之前的研究一起强调了乙酸盐和氢气是主要的甲烷前体，并且产甲烷菌是这种富营养化湖泊沉积物中主要的氢消耗者。
