用于苜蓿根瘤菌及相关细菌插入诱变的自杀质粒载体。
Suicide plasmid vehicles for insertion mutagenesis in Rhizobium meliloti and related bacteria.
作者信息
Selvaraj G, Iyer V N
出版信息
J Bacteriol. 1983 Dec;156(3):1292-300. doi: 10.1128/jb.156.3.1292-1300.1983.
Abstract
We describe the construction and use of a set of plasmid vectors of the transposons Tn1, Tn5, and Tn9 that are suicidal in Rhizobium species and therefore suitable for mutagenesis with these three transposons. The vectors are composed of the p15A replicon which functions in Escherichia coli but not in Rhizobium species and a region encoding the N type of bacterial conjugation system which is very efficient in matings between E. coli and Rhizobium species. The usefulness of the vectors has been most extensively assessed in Rhizobium meliloti. It is likely that they will be useful for mutagenesis and genome manipulation in other bacteria as well.
摘要
我们描述了转座子Tn1、Tn5和Tn9的一组质粒载体的构建及用途，这些载体在根瘤菌属中具有自杀性，因此适用于用这三种转座子进行诱变。这些载体由在大肠杆菌中起作用但在根瘤菌属中不起作用的p15A复制子，以及编码N型细菌接合系统的区域组成，该系统在大肠杆菌和根瘤菌属之间的交配中非常高效。这些载体的实用性已在苜蓿根瘤菌中得到了最广泛的评估。它们很可能也可用于其他细菌的诱变和基因组操作。
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本文引用的文献
1
Genetic transformation of Rhizobium meliloti by plasmid DNA.用质粒DNA对苜蓿根瘤菌进行遗传转化。
Gene. 1981 Nov;15(2-3):279-83. doi: 10.1016/0378-1119(81)90137-2.
2
A dnaB analog function specified by bacteriophage P7 and its comparison to the similar function specified by bacteriophage P1.噬菌体P7所指定的dnaB类似功能及其与噬菌体P1所指定的类似功能的比较。
Mol Gen Genet. 1980;178(3):561-6. doi: 10.1007/BF00337861.
3
Inverted repeats in the DNA of plasmid pCU1.质粒pCU1 DNA中的反向重复序列。
J Bacteriol. 1983 Mar;153(3):1502-12. doi: 10.1128/jb.153.3.1502-1512.1983.
4
Cloning of a plasmid region specifying the N transfer system of bacterial conjugation in Escherichia coli.大肠杆菌中决定细菌接合作用N转移系统的质粒区域的克隆
Gene. 1983 Mar;21(3):227-36. doi: 10.1016/0378-1119(83)90006-9.
5
Localization of symbiotic mutations in Rhizobium meliloti.苜蓿根瘤菌中共生突变的定位
J Bacteriol. 1983 Feb;153(2):635-43. doi: 10.1128/jb.153.2.635-643.1983.
6
Directed transposon Tn5 mutagenesis and complementation analysis of Rhizobium meliloti symbiotic nitrogen fixation genes.苜蓿根瘤菌共生固氮基因的定向转座子Tn5诱变及互补分析
Cell. 1982 Jun;29(2):551-9. doi: 10.1016/0092-8674(82)90171-4.
7
Transposable elements in prokaryotes.原核生物中的转座元件。
Annu Rev Genet. 1981;15:341-404. doi: 10.1146/annurev.ge.15.120181.002013.
8
Physical and genetic characterization of symbiotic and auxotrophic mutants of Rhizobium meliloti induced by transposon Tn5 mutagenesis.由转座子Tn5诱变诱导的苜蓿根瘤菌共生和营养缺陷型突变体的物理和遗传特征分析
J Bacteriol. 1982 Jan;149(1):114-22. doi: 10.1128/jb.149.1.114-122.1982.
9
Physical and genetic organization of the IncN-group plasmid pCU1.IncN 群质粒 pCU1 的物理和遗传组织
Gene. 1981 Aug;14(3):195-204. doi: 10.1016/0378-1119(81)90115-3.
10
A rapid boiling method for the preparation of bacterial plasmids.一种制备细菌质粒的快速煮沸法。
Anal Biochem. 1981 Jun;114(1):193-7. doi: 10.1016/0003-2697(81)90473-5.
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