用于苜蓿根瘤菌及相关细菌插入诱变的自杀质粒载体。
Suicide plasmid vehicles for insertion mutagenesis in Rhizobium meliloti and related bacteria.
作者信息
Selvaraj G, Iyer V N
出版信息
J Bacteriol. 1983 Dec;156(3):1292-300. doi: 10.1128/jb.156.3.1292-1300.1983.
Abstract
We describe the construction and use of a set of plasmid vectors of the transposons Tn1, Tn5, and Tn9 that are suicidal in Rhizobium species and therefore suitable for mutagenesis with these three transposons. The vectors are composed of the p15A replicon which functions in Escherichia coli but not in Rhizobium species and a region encoding the N type of bacterial conjugation system which is very efficient in matings between E. coli and Rhizobium species. The usefulness of the vectors has been most extensively assessed in Rhizobium meliloti. It is likely that they will be useful for mutagenesis and genome manipulation in other bacteria as well.
摘要
我们描述了转座子Tn1、Tn5和Tn9的一组质粒载体的构建及用途，这些载体在根瘤菌属中具有自杀性，因此适用于用这三种转座子进行诱变。这些载体由在大肠杆菌中起作用但在根瘤菌属中不起作用的p15A复制子，以及编码N型细菌接合系统的区域组成，该系统在大肠杆菌和根瘤菌属之间的交配中非常高效。这些载体的实用性已在苜蓿根瘤菌中得到了最广泛的评估。它们很可能也可用于其他细菌的诱变和基因组操作。
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