Institute of Biotechnology, ETH-Hönggerberg, CH-8093 Zürich, Switzerland.
Appl Environ Microbiol. 1994 Jun;60(6):2107-12. doi: 10.1128/aem.60.6.2107-2112.1994.
Actinomycetes isolated from 10 different soil and compost samples were screened for production of pectinolytic enzyme activities when grown on pectin-containing solid and liquid media. Pectinolytic enzymes, detected by using plate diffusion tests with a medium containing ramie (Boehmeria nivea) plant material as the sole carbon source, were mainly pectate lyases, but low activities of pectinesterases were also observed. Polygalacturonases and polymethylgalacturonases were not produced. Multiple forms of pectate lyases were detected in the culture supernatants of some of the strains by using the zymogram technique of isoelectric focusing gels. Xylanolytic and cellulolytic activities were always found to be associated with pectinolytic activities. None of the pectinolytic enzymes were produced in a medium with glucose as the sole carbon source. Treatment of ramie bast fibers with crude enzyme preparations from a selection of strains showed a good correlation between the pectate lyase activity applied and the degumming effect, resulting in good separation of the bast fibers.
从 10 种不同的土壤和堆肥样本中分离出的放线菌，在含有果胶的固体和液体培养基上生长时，会筛选出产生果胶酶活性的物质。通过使用含有苎麻（Boehmeria nivea）植物材料作为唯一碳源的培养基进行平板扩散试验，检测到果胶酶活性，主要是果胶裂解酶，但也观察到低活性的果胶酯酶。没有检测到多聚半乳糖醛酸酶和聚甲基半乳糖醛酸酶。在一些菌株的培养上清液中，使用等电聚焦凝胶的同工酶技术检测到了多种形式的果胶裂解酶。木聚糖酶和纤维素酶活性总是与果胶酶活性相关联。在以葡萄糖为唯一碳源的培养基中，没有产生任何果胶酶。用来自一些菌株的粗酶制剂处理苎麻韧皮纤维，应用的果胶裂解酶活性与脱胶效果之间存在很好的相关性，从而使韧皮纤维得到很好的分离。
