Department of Microbiology and Center for Biological Resource Recovery, University of Georgia, Athens, Georgia 30602.
Appl Environ Microbiol. 1994 Nov;60(11):4182-5. doi: 10.1128/aem.60.11.4182-4185.1994.
Reversible conversion of 4-hydroxybenzoate and phenol and their analogs was observed in whole-cell suspensions and cell extracts of Clostridium hydroxybenzoicum grown with 4-hydroxybenzoate and 3,4-dihydroxybenzoate. Assuming that bicarbonate is the cosubstrate, the equilibrium constants calculated for the reactions 4-hydroxybenzoate + H(2)O left arrow over right arrow phenol + HCO(3) and 3,4-dihydroxybenzoate + H(2)O left arrow over right arrow catechol + HCO(3) were 11.4 (+/- 0.5) and 5.05 (+/- 0.25), respectively. In a phenol-adapted sediment slurry, 4-hydroxybenzoate and 3,4-dihydroxybenzoate were decarboxylated to phenol and to catechol, respectively, as intermediates without a lag time.
在以 4-羟基苯甲酸和 3,4-二羟基苯甲酸为碳源培养的产羟基苯甲酸梭菌的全细胞悬液和细胞提取物中观察到 4-羟基苯甲酸和苯酚及其类似物的可逆转化。假设碳酸氢盐是共底物，计算出的反应 4-羟基苯甲酸 + H2O ⇌ 苯酚 + HCO3- 和 3,4-二羟基苯甲酸 + H2O ⇌ 儿茶酚 + HCO3- 的平衡常数分别为 11.4(+/-0.5)和 5.05(+/-0.25)。在适应苯酚的沉积物泥浆中，4-羟基苯甲酸和 3,4-二羟基苯甲酸分别在没有滞后时间的情况下脱羧生成苯酚和儿茶酚作为中间产物。
