Robinson D M, Berman S, Lowenthal J P, Hetrick F M
Department of Biologics Research, Division of Veterinary Medicine, Walter Reed Army Institute of Research, Washington, D.C.
Appl Microbiol. 1966 Nov;14(6):1011-4. doi: 10.1128/am.14.6.1011-1014.1966.
A method was developed for production of a freeze-dried Western equine encephalomyelitis vaccine from virus propagated in chick embryo cell culture monolayers maintained with a serum-free medium. A sufficient concentration of virus accumulated in the cell culture fluids prior to the occurrence of viral cytopathology to permit the production of a vaccine relatively free from serum and cellular proteins. Inoculation with two mouse ld(50) doses of virus per 100 tissue culture cells was found to yield reproducible high virus titers at a convenient harvest time. These harvests were inactivated at 22 C by 0.05% formalin within 48 hr. Potency test results, as measured by the protection of immunized guinea pigs against an intracerebral virus challenge, indicated that the vaccine produced from the virus propagated in cell culture was equal in potency to a lot of whole chick embryo vaccine used to immunize laboratory and field workers subject to a high risk of infection.
开发了一种从在无血清培养基中维持的鸡胚细胞培养单层中繁殖的病毒生产冻干西方马脑脊髓炎疫苗的方法。在病毒细胞病变出现之前，细胞培养液中积累了足够浓度的病毒，从而能够生产出相对不含血清和细胞蛋白的疫苗。发现每100个组织培养细胞接种两个小鼠半数致死剂量（ld50）的病毒，可在方便的收获时间产生可重复的高病毒滴度。这些收获物在48小时内于22℃用0.05%福尔马林灭活。通过免疫豚鼠抵抗脑内病毒攻击的保护作用来衡量的效力测试结果表明，由细胞培养中繁殖的病毒生产的疫苗效力与用于免疫面临高感染风险的实验室和现场工作人员的一批全鸡胚疫苗相当。
