Robinson D M, Berman S, Lowenthal J P
Appl Microbiol. 1972 Jan;23(1):104-7. doi: 10.1128/am.23.1.104-107.1972.
A potency assay for Western equine encephalomyelitis vaccine was developed which utilized mice as the test animal instead of guinea pigs or hamsters. By immunizing several groups of mice with dilutions of the vaccine and challenging them intracerebrally with virulent virus, it was possible to determine mathematically a dose of vaccine capable of protecting 50% of the animals (ED(50)). When log dilutions of virulent virus were used to challenge mice which were immunized with dilutions of the vaccine, there was no difference among the ED(50) values for the dilutions of challenge virus. In a direct comparison of ED(50) values determined from the immunization of mice and those determined from the immunization of guinea pigs, there were no differences in the rankings of the vaccines.
开发了一种针对西部马脑脊髓炎疫苗的效价测定方法，该方法使用小鼠作为试验动物，而非豚鼠或仓鼠。通过用疫苗稀释液免疫几组小鼠，并对它们进行脑内强毒病毒攻击，能够通过数学方法确定能保护50%动物的疫苗剂量（半数有效剂量[ED(50)]）。当用对数稀释的强毒病毒攻击用疫苗稀释液免疫的小鼠时，攻击病毒稀释液的ED(50)值之间没有差异。在直接比较从小鼠免疫测定的ED(50)值和从豚鼠免疫测定的ED(50)值时，疫苗的排名没有差异。
