在鸡胚细胞滚瓶培养物中生产的甲醛灭活委内瑞拉马脑炎（特立尼达株）疫苗。
Formalin-inactivated Venezuelan equine encephalomyelitis (Trinidad strain) vaccine produced in rolling-bottle cultures of chicken embryo cells.
作者信息
Cole F E, May S W, Robinson D M
出版信息
Appl Microbiol. 1973 Feb;25(2):262-5. doi: 10.1128/am.25.2.262-265.1973.
Abstract
Formalin-inactivated Venezuelan equine encephalomyelitis vaccine was prepared from virus grown in rolling-bottle cultures of chicken embryo cells. Trinidad strain virus was propagated in these cultures with a maintenance medium consisting of serum-free medium 199 containing 0.25% human serum albumin (USP) and antibiotics. Manipulation of multiplicity of inoculum (0.06 to 0.00006) and maintenance medium volume (100 to 300 ml) resulted in high-titered virus yields and only moderate cell destruction when fluids from infected cultures were harvested at 18 to 24 hr. The virus was inactivated at 37 C by 0.05% Formalin within 8 to 10 hr and with 0.1% Formalin within 6 to 8 hr. Single dose, antigen extinction tests in mice performed with 30 small-scale vaccine lots showed excellent potency at either Formalin concentration with inactivation periods ranging from 24 to 96 hr.
摘要
福尔马林灭活的委内瑞拉马脑炎疫苗是用在鸡胚细胞滚瓶培养物中生长的病毒制备的。特立尼达毒株病毒在这些培养物中繁殖，维持培养基由含0.25%人血清白蛋白（美国药典级）和抗生素的无血清培养基199组成。对接种复数（0.06至0.00006）和维持培养基体积（100至300毫升）进行调控，当在18至24小时收获感染培养物的液体时，可产生高滴度的病毒产量且只有适度的细胞破坏。病毒在37℃下用0.05%福尔马林在8至10小时内灭活，用0.1%福尔马林在6至8小时内灭活。用30个小规模疫苗批次在小鼠中进行的单剂量抗原灭活试验表明，在灭活期为24至96小时的情况下，两种福尔马林浓度下疫苗效力均极佳。
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