Cole F E, May S W, Eddy G A
Appl Microbiol. 1974 Jan;27(1):150-3. doi: 10.1128/am.27.1.150-153.1974.
Formalin-inactivated Venezuelan equine encephalomyelitis vaccine was prepared from virus propagated in rolling-bottle cultures of chicken embryo cells. The attenuated, TC-83 strain of virus was propagated in these cultures with a maintenance medium consisting of serum-free medium 199 containing 0.25% human serum albumin and antibiotics. By adjustment of maintenance medium volume (100 to 300 ml) and multiplicity of inoculum (0.04 to 0.00004), high-titered yields of virus were obtained with minimal cell destruction at convenient harvest times, viz, 18 to 24 h postinoculation. Inactivation of virus at 37 C was complete between 8 to 10 h with 0.05% Formalin and within 6 to 8 h with 0.1% Formalin. Antigen extinction potency tests in mice indicated that potent vaccines could be produced at both Formalin concentrations and, furthermore, that potency was not adversely affected by inactivation periods of up to 96 h.
福尔马林灭活的委内瑞拉马脑炎疫苗是由在鸡胚细胞滚瓶培养物中增殖的病毒制备而成。减毒的TC - 83病毒株在这些培养物中增殖，维持培养基由含有0.25%人血清白蛋白和抗生素的无血清培养基199组成。通过调整维持培养基体积（100至300毫升）和接种物的感染复数（0.04至0.00004），在方便的收获时间（即接种后18至24小时）以最小的细胞破坏获得了高滴度的病毒产量。在37℃下，用0.05%福尔马林8至10小时可使病毒完全灭活，用0.1%福尔马林6至8小时可使病毒完全灭活。小鼠抗原效价测试表明，两种福尔马林浓度均可生产出高效疫苗，此外，长达96小时的灭活时间对效价没有不利影响。
