Shuler M L, Aris R, Tsuchiya H M
Department of Microbiology, University of Minnesota, Minneapolis, Minnesota 55455.
Appl Microbiol. 1972 Sep;24(3):384-8. doi: 10.1128/am.24.3.384-388.1972.
The technique of hydrodynamic focusing, used to improve the resolution of the Coulter counter for the sizing of bacteria, was examined. Latex particles of 0.26 mum to 6.7 mum volume were used to examine the characteristics of the system with and without hydrodynamic focusing. The system then was evaluated for sizing mixed bacterial populations as well as single populations. Possible applications are also discussed.
研究了用于提高库尔特计数器对细菌大小测定分辨率的流体动力聚焦技术。使用体积为0.26微米至6.7微米的乳胶颗粒来研究有无流体动力聚焦时系统的特性。然后评估该系统对混合细菌群体以及单一细菌群体的大小测定能力。还讨论了可能的应用。