Fleming H P, Etchells J L, Costilow R N
Food Fermentation Laboratory, Agricultural Research Service, U. S. Department of Agriculture, and North Carolina Agricultural Experiment Station, Raleigh, North Carolina 27607.
Appl Microbiol. 1975 Dec;30(6):1040-2. doi: 10.1128/am.30.6.1040-1042.1975.
We reported earlier that Pediococcus cerevisiae FBB-61 inhibited Lactobacillus plantarum FBB-67 in mixed species inoculation used for the fermentation of brined cucumbers. Herein, 16 isolates of the Pediococcus genus from various sources were tested for inhibitory activity against L. plantarum and other microorganisms by a seeded-agar screening technique. Only two of the 16 isolates gave consistent and distinctive zones of inhibition, and both were isolated from fermenting cucumber brines on separate occasions. These two isolates did not inhibit each other but did inhibit the other 14 Pediococcus isolates in addition to L. plantarum. They also inhibited several other gram-positive bacteria, but not four species each of gram-negative bacteria and yeasts tested. Inoculation of cucumber juice broth with P. cerevisiae FBB-61 and L. plantarum WSO resulted in a drastic reduction in the plate count of L. plantarum WSO during day 1, but counts increased rapidly thereafter. Consequently, acid production by L. plantarum WSO was delayed. Noninhibitory isolates of Pediococcus had no appreciable effect on growth and acid production by L. plantarum WSO.
我们之前报道过，在用于腌制黄瓜发酵的混合菌种接种中，酿酒片球菌FBB - 61能抑制植物乳杆菌FBB - 67。在此，通过琼脂接种筛选技术，对来自不同来源的16株片球菌分离株针对植物乳杆菌和其他微生物的抑制活性进行了测试。16株分离株中只有两株产生了一致且明显的抑菌圈，并且这两株都是在不同时间从发酵的黄瓜卤水中分离得到的。这两株分离株彼此之间不产生抑制作用，但除了能抑制植物乳杆菌外，还能抑制其他14株片球菌分离株。它们还能抑制其他几种革兰氏阳性菌，但对所测试的4种革兰氏阴性菌和酵母菌均无抑制作用。在黄瓜汁肉汤中接种酿酒片球菌FBB - 61和植物乳杆菌WSO，在第1天植物乳杆菌WSO的平板计数急剧下降，但此后计数迅速增加。因此，植物乳杆菌WSO的产酸被延迟。片球菌的非抑制性分离株对植物乳杆菌WSO的生长和产酸没有明显影响。
