Haines W C, Harmon L G
Appl Microbiol. 1973 Mar;25(3):436-41. doi: 10.1128/am.25.3.436-441.1973.
Representative strains of 15 species of lactic acid bacteria were examined for their ability to influence growth of Staphylococcus aureus and production of enterotoxin in associative culture. Among the organisms used as effectors the streptococci were most inhibitory, followed by Pediococcus cerevisiae. The lactobacilli and Leuconostoc citrovorum were not inhibitory to growth and only slightly inhibitory to production of enterotoxin. Enterotoxin was detected in all cultures in which the population of S. aureus reached 8 x 10(7) per ml. At lower S. aureus populations no enterotoxin was detected after incubation for 48 h. Mechanisms of inhibition of growth and enterotoxin production by S. aureus strain 243 grown in association with Streptococcus lactis A64 or P. cerevisiae 10791 in APT broth were investigated. Competition for vital nutrients, especially niacin and biotin, and probably production of hydrogen peroxide contribute to inhibition. Production of lactic acid appears to inhibit growth of S. aureus in the early but not the late stages of incubation.
检测了15种乳酸菌的代表性菌株在混合培养中影响金黄色葡萄球菌生长及肠毒素产生的能力。在用作效应菌的微生物中，链球菌的抑制作用最强，其次是酿酒片球菌。乳酸杆菌和嗜柠檬酸明串珠菌对生长无抑制作用，对肠毒素产生仅有轻微抑制作用。在所有金黄色葡萄球菌菌量达到每毫升8×10⁷的培养物中均检测到了肠毒素。在较低的金黄色葡萄球菌菌量下，培养48小时后未检测到肠毒素。研究了在APT肉汤中与乳酸链球菌A64或酿酒片球菌10791混合培养的金黄色葡萄球菌菌株243生长及肠毒素产生的抑制机制。对重要营养物质（尤其是烟酸和生物素）的竞争，以及可能产生的过氧化氢都有助于产生抑制作用。乳酸的产生似乎在培养早期而非后期抑制金黄色葡萄球菌的生长。
