Kenny D J, Brown J M, Palmer M E, Snel M F, Bateman R H
Waters Corporation, MS Technologies Center, Floats Road, M23 9LZ, Wythenshawe, Manchester, UK.
J Am Soc Mass Spectrom. 2006 Jan;17(1):60-66. doi: 10.1016/j.jasms.2005.09.006. Epub 2005 Dec 15.
We present a novel enhancement to matrix-assisted laser desorption ionization (MALDI) post-source decay (PSD) analysis whereby fragment ions from multiple precursor ions are acquired into the same spectrum without employing a timed ion gate to preselect each parent ion. Fragment ions are matched to their corresponding precursor ions by comparing spectra acquired at slightly different reflectron electric fields. By measuring the difference in time-of-flight (TOF) between the two spectra for each fragment, it is possible to calculate the mass of the fragment ion and its parent. This new "parallel PSD" technique reduces analysis time and consumes less sample than conventional PSD, which requires an ion gate for serial preselection of precursor ions.
我们提出了一种对基质辅助激光解吸电离（MALDI）源后衰变（PSD）分析的新颖改进方法，即无需使用定时离子门预先选择每个母离子，就能将多个前体离子产生的碎片离子采集到同一个光谱中。通过比较在稍有不同的反射电场下采集的光谱，可将碎片离子与其相应的前体离子进行匹配。通过测量每个碎片在两个光谱之间的飞行时间（TOF）差异，就能计算出碎片离子及其母离子的质量。这种新的“并行PSD”技术与传统PSD相比，减少了分析时间且消耗的样品更少，传统PSD需要一个离子门来对前体离子进行串行预选。
