Lennon A M, Buchalla W, Brune L, Zimmermann O, Gross U, Attin T
Department of Operative Dentistry, Preventive Dentistry and Periodontology, Georg August University Gottingen, Germany.
Caries Res. 2006;40(1):2-5. doi: 10.1159/000088898.
Some novel caries detection and excavation devices rely on the ability of bacteria to produce red fluorescing compounds. The aim of this study was to examine the ability of selected oral microorganisms to emit red fluorescence. Streptococcus mutans, S. oralis, S. salivarius, S. sobrinus, Lactobacillus fermentans, L. casei, L. rhamnosus, Actinomyces naeslundi, A. israelii, Prevotella intermedia, and Fusobacterium nucleatum were inoculated onto Columbia agar with haemin and vitamin K and incubated anaerobically for up to 7 days in the dark. The resulting bacterial colonies were excited using filtered xenon light (405 +/- 20 nm) and digitally photographed through a 530-nm high-pass filter. The red and green portions of the colony fluorescence were analyzed using a computer program and the red/green ratio was calculated. All colonies emitted both red and green fluorescence. The green outweighed the red portion for the following species (in descending order) S. oralis, S. salivarius, S. mutans, F. nucleatum and S. sobrinus. The red portion was higher for the following species (in descending order) P. intermedia, A. naeslundi, A. israelii, L. fermentans, L. rhamnosus and L. casei. With all the bacteria examined, one color portion generally outweighed the other, giving the visual impression of either red or green fluorescence. We conclude that red fluorescence is well suited to detection of the bacteria which cause dentin caries but it is not suitable as an indicator of the presence and activity of the streptococci involved in initial caries.
一些新型龋齿检测和挖掘装置依赖于细菌产生红色荧光化合物的能力。本研究的目的是检测特定口腔微生物发出红色荧光的能力。将变形链球菌、口腔链球菌、唾液链球菌、远缘链球菌、发酵乳杆菌、干酪乳杆菌、鼠李糖乳杆菌、内氏放线菌、衣氏放线菌、中间普氏菌和具核梭杆菌接种到含血红素和维生素K的哥伦比亚琼脂上，在黑暗中厌氧培养长达7天。使用过滤后的氙灯（405±20nm）激发所得细菌菌落，并通过530nm高通滤光片进行数码拍照。使用计算机程序分析菌落荧光的红色和绿色部分，并计算红/绿比率。所有菌落均发出红色和绿色荧光。对于以下菌种（按降序排列），绿色荧光超过红色荧光：口腔链球菌、唾液链球菌、变形链球菌、具核梭杆菌和远缘链球菌。对于以下菌种（按降序排列），红色荧光部分较高：中间普氏菌、内氏放线菌、衣氏放线菌、发酵乳杆菌、鼠李糖乳杆菌和干酪乳杆菌。在所检测的所有细菌中，一种颜色部分通常超过另一种颜色部分，给人以红色或绿色荧光的视觉印象。我们得出结论，红色荧光非常适合检测导致牙本质龋的细菌，但不适合作为初始龋中涉及的链球菌存在和活性的指标。
