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暴露于405纳米激光下的特定念珠菌菌株中的红色/橙色自发荧光。

Red/Orange Autofluorescence in Selected Candida Strains Exposed to 405 nm Laser Light.

作者信息

Wiench Rafał, Paliga Dariusz, Mertas Anna, Bobela Elżbieta, Kuśka-Kiełbratowska Anna, Bordin-Aykroyd Sonia, Kawczyk-Krupka Aleksandra, Grzech-Leśniak Kinga, Lukomska-Szymanska Monika, Lynch Edward, Skaba Dariusz

机构信息

Department of Periodontal Diseases and Oral Mucosa Diseases, Faculty of Medical Sciences in Zabrze, Medical University of Silesia, 40-055 Katowice, Poland.

Dental Office Reanata and Dariusz Paliga, Aleja Niepodległości 3/lok 2, 35-303 Rzeszów, Poland.

出版信息

Dent J (Basel). 2024 Feb 26;12(3):48. doi: 10.3390/dj12030048.

DOI:10.3390/dj12030048
PMID:38534272
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10969471/
Abstract

BACKGROUND

and similar species are significant pathogens in immunocompromised and hospitalized individuals, known for mucosal colonization and bloodstream/organ invasion. Many pathogenic including these species, exhibit autofluorescence (R/OF) under specific light conditions, a feature crucial for their detection.

AIM

We investigated the use of a 405 nm diode laser for the direct observation of red/orange autofluorescence of spp., common in the oral cavity, exploring its potential in health screenings.

METHODS

This study utilized cultures of spp. on Sabouraud dextrose agar with Qdot 655 and 685 for fluorescence benchmarking, illuminated using a 405 nm diode laser (continuous wave, power 250 mW, 0.0425 J/cm² fluence, 0.0014 W/cm² power density). Images were captured using a yellow-filter camera at set intervals (48 to 144 h). Visual and computational analyses evaluated the R/OF in terms of presence, intensity, coloration, and intra-colony variation.

RESULTS

Most displayed red/orange autofluorescence at all observation times, characterized by varied coloration and intra-colony distribution. Initially, there was an increase in R/OF intensity, which then stabilized in the later stages of observation.

CONCLUSIONS

The majority of the tested are capable of emitting R/OF under 405 nm laser light. This finding opens up new possibilities for integrating R/OF detection into routine dental screenings for spp.

摘要

背景

以及类似物种是免疫功能低下和住院患者中的重要病原体，以粘膜定植和血液/器官侵袭而闻名。许多包括这些物种在内的致病微生物在特定光照条件下表现出自发荧光（R/OF），这一特征对其检测至关重要。

目的

我们研究了使用405nm二极管激光直接观察口腔中常见的 物种的红色/橙色自发荧光，探索其在健康筛查中的潜力。

方法

本研究利用在含有Qdot 655和685的沙氏葡萄糖琼脂上培养的 物种进行荧光基准测试，使用405nm二极管激光（连续波，功率250mW，通量0.0425J/cm²，功率密度0.0014W/cm²）照射。使用黄色滤光片相机在设定的时间间隔（48至144小时）拍摄图像。视觉和计算分析从存在、强度、颜色和菌落内变化方面评估了R/OF。

结果

大多数 在所有观察时间都显示出红色/橙色自发荧光，其特征是颜色和菌落内分布各不相同。最初，R/OF强度增加，然后在观察后期稳定下来。

结论

大多数测试的 能够在405nm激光下发出R/OF。这一发现为将R/OF检测纳入 物种的常规牙科筛查开辟了新的可能性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7b8/10969471/845b62123355/dentistry-12-00048-g012.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7b8/10969471/dd843afa8be6/dentistry-12-00048-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7b8/10969471/9a4f88f0988b/dentistry-12-00048-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7b8/10969471/bbc19ca1e1bf/dentistry-12-00048-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7b8/10969471/3a39b6d1608f/dentistry-12-00048-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7b8/10969471/a21abbda1947/dentistry-12-00048-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7b8/10969471/e97e0c4abbd1/dentistry-12-00048-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7b8/10969471/ce6ee74bef0c/dentistry-12-00048-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7b8/10969471/a181915fbec8/dentistry-12-00048-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7b8/10969471/b394592907ad/dentistry-12-00048-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7b8/10969471/327f108bc203/dentistry-12-00048-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7b8/10969471/afac48f29834/dentistry-12-00048-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7b8/10969471/845b62123355/dentistry-12-00048-g012.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7b8/10969471/dd843afa8be6/dentistry-12-00048-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7b8/10969471/9a4f88f0988b/dentistry-12-00048-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7b8/10969471/bbc19ca1e1bf/dentistry-12-00048-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7b8/10969471/3a39b6d1608f/dentistry-12-00048-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7b8/10969471/a21abbda1947/dentistry-12-00048-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7b8/10969471/e97e0c4abbd1/dentistry-12-00048-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7b8/10969471/ce6ee74bef0c/dentistry-12-00048-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7b8/10969471/a181915fbec8/dentistry-12-00048-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7b8/10969471/b394592907ad/dentistry-12-00048-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7b8/10969471/327f108bc203/dentistry-12-00048-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7b8/10969471/afac48f29834/dentistry-12-00048-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7b8/10969471/845b62123355/dentistry-12-00048-g012.jpg

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