Ota F, Ota M, Mahmud Z H, Mohammad A, Yamato M, Kassu A, Kato Y, Tomotake H, Batoni G, Campa M
Department of Preventive Environment and Nutrition, Institute of Health Biosciences, University of Tokushima, Tokushima-shi, Japan.
Caries Res. 2006;40(1):6-14. doi: 10.1159/000088899.
A set of monoclonal antibodies were prepared by the conventional cell fusion of myeloma cells (SP2/0-Ag14) with spleen cells from BALB/c mice immunised with whole cells of a strain of mutans streptococci. Their specificities were examined against 35 reference strains of mutans streptococci, 34 reference strains of other oral streptococci and 8 reference strains of other microorganisms often inhabiting the oral cavity. Specificity was examined by enzyme immunoassay using whole cells. A total of 52 strains, consisting of 19 strains isolated in Japan, 19 strains isolated in Italy and 14 strains isolated in England, were characterised by conventional physiological and biochemical tests and then serotyped by the use of 8 monoclonal antibodies with different specificities. They were also confirmed by guanine-plus-cytosine contents of their nucleic acid and DNA-DNA hybridisation test. The results indicated that all monoclonal antibodies are useful for identification of 8 serotypes of the mutans streptococci responsible for dental caries. They also suggest the existence of more serological varieties among mutans species.
通过骨髓瘤细胞（SP2/0-Ag14）与用变形链球菌菌株全细胞免疫的BALB/c小鼠脾细胞进行常规细胞融合，制备了一组单克隆抗体。针对35株变形链球菌参考菌株、34株其他口腔链球菌参考菌株以及8株常见于口腔的其他微生物参考菌株，检测了它们的特异性。使用全细胞通过酶免疫测定法检测特异性。共有52株菌株，其中19株在日本分离、19株在意大利分离、14株在英国分离，通过常规生理生化试验进行了鉴定，然后使用8种具有不同特异性的单克隆抗体进行血清分型。还通过核酸的鸟嘌呤加胞嘧啶含量和DNA-DNA杂交试验进行了确认。结果表明，所有单克隆抗体都可用于鉴定导致龋齿的8种变形链球菌血清型。它们还表明变形链球菌种内存在更多血清学变种。
