用单克隆抗体鉴定变形链球菌

Identification of mutans streptococci with monoclonal antibodies.

作者信息

de Soet J J, van Dalen P J, Russell R R, de Graaff J

机构信息

Department of Oral Microbiology, Academic Centre for Dentistry, Amsterdam ACTA, The Netherlands.

出版信息

Antonie Van Leeuwenhoek. 1990 Nov;58(4):219-25. doi: 10.1007/BF00399332.

DOI:10.1007/BF00399332

PMID:1707251

Abstract

Mutans streptococci have been correlated with dental caries. The identification of the species within this group is still a problem. The characterization of a monoclonal antibody (Mab) OMVU10 against S. sobrinus as well as the isolation and characterization of Mabs against S. mutans (OMVU30 and OMVU31), S. cricetus (OMVU40) and mutans streptococci (OMVU2) is described. The epitope specificity for OMVU10 and OMVU31 was cell-wall antigen B in both cases although both Mabs recognized different species-specific epitopes. OMVU40 was cross reactive with Streptococcus sanguis taxon 3. All other Mabs were specific for one species. Using these Mabs, a key to the identification of mutans streptococci is developed. This key was tested for 85 wild type isolates of mutans streptococci and proved to be highly reliable and easy to perform.

摘要

变形链球菌与龋齿有关。该菌群内各菌种的鉴定仍是一个问题。本文描述了一种针对远缘链球菌的单克隆抗体（Mab）OMVU10的特性，以及针对变形链球菌（OMVU30和OMVU31）、仓鼠链球菌（OMVU40）和变形链球菌群（OMVU2）的单克隆抗体的分离和特性。尽管这两种单克隆抗体识别不同的种特异性表位，但OMVU10和OMVU31的表位特异性在两种情况下均为细胞壁抗原B。OMVU40与血链球菌分类群3有交叉反应。所有其他单克隆抗体对一种菌种具有特异性。利用这些单克隆抗体，开发了一种变形链球菌鉴定方法。该方法对85株变形链球菌野生型菌株进行了测试，结果证明高度可靠且易于操作。