Ota F, Hirota K, Nagamune H, Fukui K
School of Dentistry, The University of Tokushima, Japan.
Dent Jpn (Tokyo). 1990;27(1):25-8.
Monoclonal antibodies (MAbs) were prepared from myeloma cells and splenocytes of mice immunized with Streptococcus cricetus HS1 or Streptococcus downei MF25 cells. Various makes of immunoplates were coated with whole cells or purified polysaccharide antigens of oral streptococcal strains and tested for reactivity in enzyme immunoassay (EIA) using the Mabs. It was revealed that different immunoplates had different reactivities. It was also shown that the EIA system and MAbs were useful for the immunological study and identification of oral streptococci.
用仓鼠链球菌HS1或唐氏链球菌MF25细胞免疫小鼠，从骨髓瘤细胞和脾细胞中制备单克隆抗体（MAbs）。将各种品牌的免疫板用口腔链球菌菌株的全细胞或纯化的多糖抗原包被，并使用单克隆抗体在酶免疫测定（EIA）中检测反应性。结果显示，不同的免疫板具有不同的反应性。还表明，EIA系统和单克隆抗体可用于口腔链球菌的免疫学研究和鉴定。
