Basova N A, Markov Iu G, Berzinia N I
Ross Fiziol Zh Im I M Sechenova. 2005 Sep;91(9):1097-107.
Membrane-bound serine proteases to play a certain role in activation of sodium transport in epithelial cells. To were found explain the protease activity and sodium-dependent L-tryptophan transport across chicken small intestine interaction, four experiments were conducted. One hundred chicks were fed diets that contained 0; 0.3; 3 or 6% of supplemental NaCl and were given distillated water ad libitum. Signs of salt toxicity observed were as follows: a decreased body weight, increased heart and kidney weights, formation of secondary lysosomes in enterocytes and lymphocytes. Such chickens were in the state of negative nitrogen balance. Intestinal absorption of L-tryptophan correlated with mucosal protease activity during increased dietary sodium chloride intake. Recent in vitro and in vivo experiments indicate that enterocyte proteases may be of critical importance in activation of sodium-dependent intestinal transporters for L-tryptophan.
膜结合丝氨酸蛋白酶在上皮细胞钠转运激活中发挥一定作用。为解释蛋白酶活性与钠依赖性L-色氨酸跨鸡小肠转运之间的相互作用，进行了四项实验。100只雏鸡被喂食含0%、0.3%、3%或6%补充氯化钠的日粮，并随意给予蒸馏水。观察到的盐中毒迹象如下：体重减轻、心脏和肾脏重量增加、肠细胞和淋巴细胞中次级溶酶体形成。这些鸡处于负氮平衡状态。在增加日粮氯化钠摄入量期间，L-色氨酸的肠道吸收与粘膜蛋白酶活性相关。最近的体外和体内实验表明，肠细胞蛋白酶可能在激活L-色氨酸的钠依赖性肠道转运蛋白中起关键作用。
