Kushak R I, Basova N A
Fiziol Zh SSSR Im I M Sechenova. 1978 Jan;64(1):102-7.
The mechanism of peptide transport was studied in vitro in the small intestine of chicks and accumulating preparations of mucosa using glycyl-1-tryptophan. A special criterion was proposed to differentiate the processes of intracellular and membrane hydrolysis of dipeptide basing on the analysis of transport intensity of amino acids released into the serosal solution after hydrolysis of dipeptide. 1-tryptophan released at hydrolysis was transported considerably more slowly than free amino acid. 1-tryptophan from the mixture with glycyne demonstrated the highest transport intensity in serosal solution as well as the highest accumulation in the mucosa cells from all forms of 1-tryptophan. At the same time the peptide form of glycyne was transported with the same intensity as that from the mixture with 1-tryptophan. According to the criterion applied it is supposed that unequal levels of 1-tryptophan and glycyne in the serosal solution prove the membrane hydrolysis of glycyne-1-tryptophan during ist transport in enterocytes.
利用甘氨酰-1-色氨酸在雏鸡小肠和黏膜累积制剂中对肽转运机制进行了体外研究。基于对二肽水解后释放到浆膜溶液中的氨基酸转运强度的分析，提出了一个区分二肽细胞内水解和膜水解过程的特殊标准。水解时释放的1-色氨酸的转运速度比游离氨基酸慢得多。来自与甘氨酸混合物中的1-色氨酸在浆膜溶液中的转运强度最高，并且在所有形式的1-色氨酸中，其在黏膜细胞中的累积量也最高。同时，甘氨酸的肽形式与来自与1-色氨酸混合物中的甘氨酸以相同的强度进行转运。根据所应用的标准推测，浆膜溶液中1-色氨酸和甘氨酸水平的不同证明了甘氨酰-1-色氨酸在肠细胞转运过程中的膜水解。
