Golub M S, Keen C L, Gershwin M E
Department of Internal Medicine, University of California, Davis 95616.
Neurotoxicol Teratol. 1992 May-Jun;14(3):177-82. doi: 10.1016/0892-0362(92)90013-z.
In order to determine sensitive periods for induction of neurodevelopmental effects of aluminum (Al), mice were fed either 25 (control) or 1000 (high Al) micrograms Al/g diet (as Al lactate) from conception through lactation and litters were fostered either within or between groups at birth. Birth parameters were not influenced by Al intake. Food intake and body weight were 10%-12% lower during lactation in dams fed the high Al diets. Both gestation and lactation high Al exposure led to growth retardation in offspring beginning on day 10 postnatal; combined gestation and lactation exposure led to the biggest weight differential at weaning (23%). For neurobehavioral measures obtained at weaning, forelimb grasp strength was influenced by gestation high Al exposure, whereas negative geotaxis was influenced by lactation exposure, and hindlimb grasp and temperature sensitivity were influenced by both gestation and lactation exposure. Pup liver and brain manganese (Mn) and liver iron (Fe) concentrations at weaning were lower after high Al lactation exposure than in controls. Pup brain and liver Al concentrations were similar among the groups. These data show that mice are susceptible to neurodevelopmental effects of high maternal dietary Al intake during both gestation and lactation, and that high maternal intake can result in altered essential trace element metabolism in the offspring.
为了确定铝（Al）诱导神经发育效应的敏感期，从受孕到哺乳期，给小鼠喂食每克饮食含25微克（对照）或1000微克（高铝）铝（以乳酸铝形式）的饲料，出生时将同窝幼崽在组内或组间寄养。出生参数不受铝摄入量的影响。喂食高铝饲料的母鼠在哺乳期的食物摄入量和体重降低了10%-12%。孕期和哺乳期高铝暴露均导致子代从出生后第10天开始生长迟缓；孕期和哺乳期联合暴露导致断奶时体重差异最大（23%）。对于断奶时获得的神经行为指标，前肢抓握力受孕期高铝暴露影响，负趋地性受哺乳期暴露影响，后肢抓握力和温度敏感性受孕期和哺乳期暴露共同影响。高铝哺乳期暴露后，断奶时幼崽肝脏和大脑中的锰（Mn）以及肝脏中的铁（Fe）浓度低于对照组。各组幼崽大脑和肝脏中的铝浓度相似。这些数据表明，小鼠在孕期和哺乳期均易受母体高膳食铝摄入量的神经发育影响，且母体高摄入量可导致子代必需微量元素代谢改变。
