Li W M, Cui K M, Li N, Gu Q B, Schwarz W, Ding G H, Wu G C
Department of Integrative Medicine, Shanghai Medical College, Fudan University, Shanghai 200032, China.
Jpn J Physiol. 2005 Dec;55(6):339-44. doi: 10.2170/jjphysiol.RP001505. Epub 2005 Dec 17.
Electroacupuncture (EA) was applied bilaterally to the acupoints of Zu-san-li (ST-36) and Kun-lun (BL-60) in the hindlimbs of mice. The therapeutic effect of EA on inflammatory pain induced by an ipsilateral injection of complete Freund's adjuvant (CFA) into the right paw of the mouse was investigated in this study. The time of paw-withdrawal latency (PWL) was used as an indicator for judging the intensity of the pain induced by the CFA injection. The EA effects were divided into immediate (PWL tests within 2 h after EA treatment) and cumulative (PWL tests during and after repetitive EA treatments for 3 weeks) effects. As immediate effects, PWL was significantly shortened in the CFA-injected paw, but was again prolonged 20 min after an EA treatment and lasted until 30 min after. As cumulative effects, PWL was significantly shortened in the CFA-injected paw, but recovered from the 2nd to the 8th day during repetitive EA treatments. No such effects could be observed after sham EA treatment, which resulted in behavior similar to that in untreated animals. These results demonstrate that the CFA-induced inflammatory pain in mice is an ideal model system for the investigation of EA effects and may serve as a valuable reference for the clinical treatment of inflammatory pain in human beings. Furthermore, the mouse pain model opens the possibility to apply the investigation also to transgenic mice.
对小鼠后肢的足三里穴（ST-36）和昆仑穴（BL-60）进行双侧电针治疗。本研究探讨了电针对小鼠右爪同侧注射完全弗氏佐剂（CFA）诱导的炎性疼痛的治疗效果。以缩爪潜伏期（PWL）作为判断CFA注射所致疼痛强度的指标。电针效应分为即时效应（电针治疗后2小时内进行PWL测试）和累积效应（重复电针治疗3周期间及之后进行PWL测试）。作为即时效应，CFA注射侧爪的PWL显著缩短，但电针治疗后20分钟再次延长，并持续至治疗后30分钟。作为累积效应，CFA注射侧爪的PWL显著缩短，但在重复电针治疗期间从第2天到第8天恢复。假电针治疗后未观察到此类效应，其行为与未治疗动物相似。这些结果表明，CFA诱导的小鼠炎性疼痛是研究电针效应的理想模型系统，可为人类炎性疼痛的临床治疗提供有价值的参考。此外，该小鼠疼痛模型为将研究也应用于转基因小鼠开辟了可能性。
