Massa G, de Zegher F, Vanderschueren-Lodeweyckx M
Department of Paediatrics, University of Leuven, Belgium.
Pediatr Res. 1992 Jul;32(1):69-72. doi: 10.1203/00006450-199207000-00013.
Growth hormone-binding protein (GH-BP) levels were studied in cord serum of 69 human infants born after 24 to 41 wk of gestation and in serum of 14 infants aged 1 to 3 mo. GH-BP levels were measured by HPLC-gel filtration of serum incubated overnight with 125I-hGH. The radioactive elution profile revealed two small 125I-hGH peaks of high molecular weight and a large peak, corresponding to monomeric 125I-hGH. The first peak of high molecular weight was variable, showed some of the characteristics (high molecular weight, displaceability by a large excess of unlabeled hGH) of the described low affinity, high capacity GH-BP, and did not correlate with gestational age or birth weight (peak I-BP). The second peak was identified as 125I-hGH bound to the high affinity, low capacity GH-BP (peak II-BP). Mean +/- SD specific binding of 125I-hGH to this peak was significantly (p less than 0.0001) different between preterm infants (3.1 +/- 1.1%; n = 51), term infants at birth (4.2 +/ 1.1%; n = 18), and 1- to 3-mo-old infants (8.5 +/- 1.6%; n = 14). To evaluate the effect of intrauterine nutritional state, the ponderal index (weight/lengths) was calculated. Peak II-BP levels were lower (p less than 0.05) in infants with the ponderal index less than 2.35 (2.8 +/- 1.0%; n = 20) than in those with the ponderal index between 2.35 and 2.65 (3.4 +/- 1.2%; n = 29) or greater than 2.65 (3.8 +/- 1.2%; n = 20).(ABSTRACT TRUNCATED AT 250 WORDS)
对69例孕24至41周出生的人类婴儿的脐带血清以及14例1至3个月大婴儿的血清中的生长激素结合蛋白（GH - BP）水平进行了研究。通过对与125I - hGH孵育过夜的血清进行高效液相色谱 - 凝胶过滤来测量GH - BP水平。放射性洗脱图谱显示出两个高分子量的小125I - hGH峰和一个对应于单体125I - hGH的大峰。第一个高分子量峰变化不定，具有所描述的低亲和力、高容量GH - BP的一些特征（高分子量、可被大量未标记hGH置换），且与胎龄或出生体重无关（峰I - BP）。第二个峰被鉴定为与高亲和力、低容量GH - BP结合的125I - hGH（峰II - BP）。早产婴儿（3.1±1.1%；n = 51）、出生时的足月儿（4.2±1.1%；n = 18）和1至3个月大婴儿（8.5±1.6%；n = 14）之间，125I - hGH与该峰的平均±标准差特异性结合存在显著差异（p＜0.0001）。为评估宫内营养状态的影响，计算了 ponderal指数（体重/身长）。ponderal指数小于2.35的婴儿（2.8±1.0%；n = 20）的峰II - BP水平低于ponderal指数在2.35至2.65之间的婴儿（3.4±1.2%；n = 29）或大于2.65的婴儿（3.8±1.2%；n = 20）（p＜0.05）。（摘要截断于250字）
