Iudinkova e S, Petrov A V, Vasetskiĭ E S, Razin S V
Mol Biol (Mosk). 2005 Nov-Dec;39(6):971-7.
We have developed a technique for mapping the sites of DNA attachment to the nuclear matrix by hybridization of nuclear matrix DNA with an oligonucleotide array. The latter was made by immobilization of 60-mer oligonucleotides distributed within the area under study with a 2 Kb step on nylon filter. Using this approach we have analyzed the mode of interaction of a 100 Kb fragment of chicken chromosome 16 including the alpha-globin gene domain with the nuclear matrix. The 40 Kb DNA loop including all alpha-globin genes was detected in erythroid cells. One of the borders of this loop colocalized with the previously mapped MAR element and CTCF-dependent enhancer-blocking element. Also, a long transcribed area was found to be preferentially associated with the nuclear matrix. The spatial organization of the area under study in lymphoid cells was drastically different from this observed in erythroid cells.
我们开发了一种技术，通过核基质DNA与寡核苷酸阵列杂交来绘制DNA附着于核基质的位点。后者是通过将分布在研究区域内、间隔2 Kb的60聚体寡核苷酸固定在尼龙滤膜上制成的。利用这种方法，我们分析了鸡16号染色体上包括α-珠蛋白基因结构域在内的100 Kb片段与核基质的相互作用模式。在红细胞中检测到包含所有α-珠蛋白基因的40 Kb DNA环。该环的一个边界与先前定位的MAR元件和CTCF依赖性增强子阻断元件共定位。此外，还发现一个长转录区域优先与核基质相关。在淋巴细胞中研究区域的空间组织与在红细胞中观察到的情况截然不同。
