Samsonova Zh V, Shchelokova O S, Ivanova N L, Rubtsova M Iu, Egorov A M
Prikl Biokhim Mikrobiol. 2005 Nov-Dec;41(6):668-75.
An indirect immunoassay for quantitative determination of ampicillin (range, 10-1000 ng/ml) in buffer or milk has been developed. Polyclonal antibodies were obtained against ampicillin conjugated with bovine serum albumin; the conjugate was synthesized by direct condensation using carbodiimide. The antibodies were specific for ampicillin and exhibited low cross-reactivity to other penicillins (azlocillin, 17%; penicillin G, 10%; piperacillin, 5%; and carbenicillin, 4%). Matrix effects were minimized by combining the use of a casein-supplemented buffer (content of casein, 1%) with sample dilution. The threshold of ampicillin detection in milk (diluted tenfold) was equal to 5.0 ng/ml (which corresponded to 50 ng/ml of the original sample).
已开发出一种间接免疫测定法，用于定量测定缓冲液或牛奶中的氨苄西林（范围为10 - 1000 ng/ml）。制备了针对与牛血清白蛋白偶联的氨苄西林的多克隆抗体；该偶联物通过使用碳二亚胺直接缩合合成。这些抗体对氨苄西林具有特异性，对其他青霉素的交叉反应性较低（阿洛西林为17%；青霉素G为10%；哌拉西林为5%；羧苄西林为4%）。通过将使用添加酪蛋白的缓冲液（酪蛋白含量为1%）与样品稀释相结合，将基质效应降至最低。牛奶（稀释十倍）中氨苄西林的检测限为5.0 ng/ml（相当于原始样品中的50 ng/ml）。
