Lipetsk State Technical University, 30 Moskovskaya St., Lipetsk 398600, Russia.
Lipetsk State Technical University, 30 Moskovskaya St., Lipetsk 398600, Russia.
Talanta. 2014 Mar;120:312-7. doi: 10.1016/j.talanta.2013.12.018. Epub 2013 Dec 18.
Piezoelectric immunosensors on the basis of homologous and group-specificantibodies have been developed for detecting penicillin G, ampicillin, and the total content of penicillin antibiotics. The receptor coating of the sensor was obtained by the immobilization of penicillin G or ampicillin hapten-protein conjugates on the polypyrrole film obtained by electropolymerization and activated by glutaraldehyde. The affinity constants and the cross reactivity coefficients have been calculated. This made it possible to estimate the affinity and specificity of the polyclonal and monoclonal antibodies used. The calibration curves are linear in the range of concentrations 2.5-250.0 ng ml(-1) (penicillin G), 2.5-500.0 ng ml(-1) (ampicillin), and 1-500 ng ml(-1) (group of penicillin). The limits of detection are 0.8 ng ml(-1), 3.9 ng ml(-1), which are lower than MRL, established for penicillin antibiotics. The sensors were tested in detecting penicillins in milk, pork, beef, liver.
基于同源和组特异性抗体的压电免疫传感器已被开发用于检测青霉素 G、氨苄西林和青霉素类抗生素的总量。传感器的受体涂层是通过将青霉素 G 或氨苄西林半抗原-蛋白缀合物固定在电聚合得到的聚吡咯膜上获得的，并通过戊二醛进行激活。计算了亲和常数和交叉反应系数。这使得可以估计所使用的多克隆和单克隆抗体的亲和力和特异性。校准曲线在浓度 2.5-250.0 ng ml(-1)（青霉素 G）、2.5-500.0 ng ml(-1)（氨苄西林）和 1-500 ng ml(-1)（青霉素组）范围内呈线性。检测限分别为 0.8 ng ml(-1)、3.9 ng ml(-1)，低于为青霉素类抗生素设定的 MRL。该传感器已在牛奶、猪肉、牛肉、肝脏中检测青霉素。
