de Reuille Pierre Barbier, Bohn-Courseau Isabelle, Godin Christophe, Traas Jan
INRA, UMR AMAP, TA40/PSII, Boulevard de la Lironde, 34398 Montpellier Cedex 5, France.
Plant J. 2005 Dec;44(6):1045-53. doi: 10.1111/j.1365-313X.2005.02576.x.
In vivo microscopy generates images that contain complex information on the dynamic behaviour of three-dimensional (3D) objects. As a result, adapted mathematical and computational tools are required to help in their interpretation. Ideally, a complete software chain to study the dynamics of a complex 3D object should include: (i) the acquisition, (ii) the preprocessing and (iii) segmentation of the images, followed by (iv) a reconstruction in time and space and (v) the final quantitative analysis. Here, we have developed such a protocol to study cell dynamics at the shoot apical meristem in Arabidopsis. The protocol uses serial optical sections made with the confocal microscope. It includes specially designed algorithms to automate the identification of cell lineage and to analyse the quantitative behaviour of the meristem surface.
体内显微镜技术生成的图像包含有关三维（3D）物体动态行为的复杂信息。因此，需要适用的数学和计算工具来辅助对其进行解读。理想情况下，用于研究复杂3D物体动态的完整软件链应包括：（i）图像采集，（ii）预处理，（iii）分割，随后是（iv）时空重建以及（v）最终的定量分析。在此，我们开发了这样一种方案来研究拟南芥茎尖分生组织中的细胞动态。该方案使用共聚焦显微镜制作的连续光学切片。它包括专门设计的算法，用于自动识别细胞谱系并分析分生组织表面的定量行为。
