Takahashi Kazuhiro, Kikuchi Kumi, Maruyama Yutaka, Urabe Tomoko, Nakajima Kiichiro, Sasano Hironobu, Imai Yutaka, Murakami Osamu, Totsune Kazuhito
Department of Analytical Medical Technology, Tohoku University School of Health Sciences, 2-1 Seiryo-machi, Aoba-ku, Sendai 980-8575, Japan.
Peptides. 2006 Jun;27(6):1383-9. doi: 10.1016/j.peptides.2005.11.004. Epub 2005 Dec 15.
Adrenomedullin 2/intermedin (AM2/IMD) is a novel member of the calcitonin/calcitonin gene-related peptide (CGRP) peptide family. AM2/IMD has a vasodilator action, and antidiuretic and antinatriuretic effects in mice. The aim of the present study is to clarify immunolocalization of AM2/IMD in human hypothalamus, heart and kidney obtained at autopsy. Immunocytochemistry showed AM2/IMD-immunoreactive cell bodies in the paraventricular and supraoptic nuclei of human hypothalamus. Both parvocellular and magnocellular cells in the paravetricular nucleus are immunostained with AM2/IMD. Immunostaining of serial sections showed co-localization of AM2/IMD-like immunoreactivity and vasopressin in the paraventricular nucleus. Myocardial cells of the heart and renal tubular cells were positively immunostained with AM2/IMD, whereas neither renal glomeruli nor vasculature in the heart and kidney were immunostained. Reverse-transcriptase polymerase chain reaction confirmed expression of AM2/IMD mRNA in the brain, pituitary, heart and kidney. The present study has shown the wide expression of AM2/IMD in human hypothalamus, heart and kidney, raising the possibility that this novel peptide may be related to the central and peripheral regulation of the circulation and water-electrolyte metabolism.
肾上腺髓质素2/中间介质（AM2/IMD）是降钙素/降钙素基因相关肽（CGRP）肽家族的一个新成员。AM2/IMD在小鼠中具有血管舒张作用以及抗利尿和抗利钠作用。本研究的目的是阐明AM2/IMD在尸检获得的人下丘脑、心脏和肾脏中的免疫定位。免疫细胞化学显示人下丘脑室旁核和视上核中有AM2/IMD免疫反应性细胞体。室旁核中的小细胞和大细胞均被AM2/IMD免疫染色。连续切片的免疫染色显示室旁核中AM2/IMD样免疫反应性与加压素共定位。心脏的心肌细胞和肾小管细胞被AM2/IMD阳性免疫染色，而心脏和肾脏中的肾小球及脉管系统均未被免疫染色。逆转录聚合酶链反应证实AM2/IMD mRNA在脑、垂体、心脏和肾脏中表达。本研究表明AM2/IMD在人下丘脑、心脏和肾脏中广泛表达，这增加了这种新肽可能与循环及水电解质代谢的中枢和外周调节相关的可能性。
